A 43-Nucleotide U-rich Element in 3′-Untranslated Region of Large Number of Trypanosoma cruzi Transcripts Is Important for mRNA Abundance in Intracellular Amastigotes*
A 43-Nucleotide U-rich Element in 3′-Untranslated Region of Large Number of Trypanosoma cruzi Transcripts Is Important for mRNA Abundance in Intracellular Amastigotes*
复制标题
大量克氏锥虫转录物 3′-非翻译区中富含 43 核苷酸 U 的元件对于细胞内无鞭毛体的 mRNA 丰度非常重要*
DOI：
--
复制
发表时间：
2012
影响因子：
4.8
通讯作者：
R. Docampo
中科院分区：
文献类型：
--
作者：
Zhu;Javier G. De Gaudenzi;V. Alvarez;Nicolás Mendiondo;Haiming Wang;J. Kissinger;A. Frasch;R. Docampo
Background: Trypanosoma cruzi regulates gene expression by means of post-transcriptional mechanisms. Results: A 43-nt U-rich element was found in the 3′-UTR of a large number of mRNAs that are more abundant in intracellular amastigotes. Conclusion: The 43-nt U-rich element might be involved in the modulation of abundance and/or translation of transcripts in amastigotes. Significance: Results suggest the existence of stage-specific RNA regulons in T. cruzi. Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation of transcription initiation and makes use of post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in the 3′-untranslated region (3′-UTR) of a large number of T. cruzi mRNAs that is important for mRNA abundance in the intracellular amastigote stage of the parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determine the transcript levels of more than 900 U-rich-containing mRNAs of large gene families as well as single and low copy number genes. Our results indicate that the 43-nt U-rich mRNA element is preferentially present in amastigotes. The cis-element of a protein kinase 3′-UTR but not its mutated version promoted the expression of the green fluorescent protein reporter gene in amastigotes. The regulatory cis-element, but not its mutated version, was also shown to interact with the trypanosome-specific RNA-binding protein (RBP) TcUBP1 but not with other related RBPs. Co-immunoprecipitation experiments of TcUBP1-containing ribonucleoprotein complexes formed in vivo validated the interaction with representative endogenous RNAs having the element. These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage.
DOI：
10.1073/pnas.83.6.1670
发表时间：
1986-03-01
影响因子：
11.1
作者：
CAPUT, D;BEUTLER, B;CERAMI, A
通讯作者：
CERAMI, A