INFECTIVITY OF THE SUBSPECIES OF THE LEISHMANIA-BRAZILIENSIS COMPLEX INVIVO AND INVITRO
INFECTIVITY OF THE SUBSPECIES OF THE LEISHMANIA-BRAZILIENSIS COMPLEX INVIVO AND INVITRO
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DOI：
10.4269/ajtmh.1990.43.623
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发表时间：
1990-12-01
影响因子：
3.3
通讯作者：
SARAVIA, NG
中科院分区：
文献类型：
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作者：
REY, JA;TRAVI, BL;SARAVIA, NG
巴西利什曼原虫传染性研究。以人巨噬细胞系U937和近交系地鼠为实验对象，测定其在Senekjie培养基中的生长动力学。在这两个系统中，每个亚种的传染性都是不同的。6日龄（静止期早期）培养的巴拿马乳杆菌原鞭毛菌的感染性最强，而8-10日龄（生长静止期后期）培养的古延乳杆菌和巴西乳杆菌的感染性最强。虽然最大的传染性发生在平稳生长期间，但没有观察到严格的生长阶段依赖性。这些培养日的寄生虫种群主要由小的、高度运动的鞭毛体组成，鞭毛是其细胞体长度的2-3倍。这些promastigotes与沙蝇媒介传播的感染形式相似。不同世卫组织参考菌株对这些亚种在仓鼠中引起的疾病具有明显的病理特征：古yanl . b.b ensis发生了最严重的病变，而分别接种巴拿马L. b.b ensis和巴西L. b.b ensis时观察到中度和不明显的病变。
The infectivity of Leishmania braziliensis ssp. in relation to their growth kinetics in Senekjie's medium was determined using the human macrophage cell line U937 and inbred hamsters. In both systems, infectivity was shown to be distinctive for each subspecies. While L. b. panamensis promastigotes from 6-day-old cultures (early stationary phase) were more infective than parasites from any other culture day, L. b. guyanensis and L. b. braziliensis reached maximum infectivity on days 8-10 and 10 (late stationary phase of growth), respectively. Although maximum infectivity occurred during stationary growth, strict growth phase dependency was not observed. The populations of parasites on these culture days were composed mostly of small, highly motile promastigotes with flagella 2-3 times the length of their cell bodies. These promastigotes resembled the infective forms transmitted by the sand fly vector. A distinct pathological picture characterized the disease caused by the different WHO reference strains for these subspecies in hamsters: L. b. guyanensis developed the most severe lesions, while moderate and inconspicuous lesions were observed when L. b. panamensis and L. b. braziliensis, respectively, constituted the inocula.