Identification of Rat Prostatic Secreted Proteins Using Mass Spectrometric Analysis and Androgen-Dependent mRNA Expression
Identification of Rat Prostatic Secreted Proteins Using Mass Spectrometric Analysis and Androgen-Dependent mRNA Expression
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DOI：
10.2164/jandrol.108.005553
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发表时间：
2009-11-01
影响因子：
--
通讯作者：
Kitamura, Shigeyuki
中科院分区：
文献类型：
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作者：
Fujimoto, Nariaki;Suzuki, Tomoharu;Kitamura, Shigeyuki
大鼠已被用于研究哺乳动物前列腺的功能和发育。为了更好地了解前列腺分泌蛋白的生理功能，鉴定前列腺分泌蛋白是重要的。以前的研究表明，前列腺素，半胱氨酸相关蛋白1，和激肽释放酶S3是在腹侧前列腺（VP），而蛋白probasin，前列腺分泌肽94，转氨酶4，和碳酸酐酶11中产生的侧前列腺，背侧前列腺（DP），和前列腺前部。当观察雄激素依赖性以及前列腺特异性表达时，它们也是有用的标志物。虽然一些大鼠前列腺蛋白已被研究，前列腺的整体蛋白质表达谱还没有被检查。在本研究中，从大鼠前列腺分泌物进行二维凝胶电泳，然后质谱分析。除了以前已知的蛋白质，蛋白质组分析揭示了几个新的分泌蛋白，包括精胺结合蛋白和类似于免疫球蛋白结合蛋白的蛋白质。此外，在DP中发现附睾分泌蛋白1和过氧化物酶6，而葡萄糖调节蛋白78在前列腺的所有叶中被确定。动物的阉割导致所有这些分泌蛋白质的mRNA减少。虽然VP中几乎完全不存在前列腺蛋白的mRNA，但其他叶中的减少有限。从我们的研究结果大鼠前列腺分泌的新观点应有助于了解前列腺的生物学功能。
Rats have been used to study the function and development of the mammalian prostate. Identification of prostatic secreted proteins is important in order to better understand their physiological function. Previous investigations have showed that prostatein, cysteine-related protein 1, and kallikrein S3 are in the ventral prostate (VP), whereas the proteins probasin, prostate secretory peptide 94, transglutaminase 4, and carbonic anhydrase 11 are produced in the lateral prostate, dorsal prostate (DP), and anterior prostate. They are also useful markers when looking at androgen dependency as well as prostate-specific expression. Although some of the rat prostatic proteins have been investigated well, the overall protein expression profile of the prostate has not been examined. In the present study, the secretions from the rat prostate were subjected to 2-dimensional gel electrophoresis followed by mass spectrometric analysis. In addition to the previously known proteins, proteome analysis revealed several new secreted proteins, including spermine-binding protein and a protein similar to immunoglobulin-binding protein. In addition, epididymal secreted protein 1 and peroxiredoxin 6 were found in the DP, while glucose-regulated protein 78 was identified in all lobes of the prostate. Castration of the animals led to a decrease in the mRNAs of all of these secreted proteins. While the mRNAs of prostatic proteins became almost completely absent in the VP, the reductions in the other lobes were limited. A novel view of rat prostate secretion from our results should contribute to an understanding of the biological functions of the prostate gland.