Zoster in children with cancer: radioimmune precipitation profiles of sera before and after illness.
Zoster in children with cancer: radioimmune precipitation profiles of sera before and after illness.
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癌症儿童带状疱疹：患病前后血清的放射免疫沉淀谱。
DOI：
10.1093/infdis/147.1.47
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Grose,C
中科院分区：
文献类型：
--
作者：
Grose,C
用放射免疫沉淀技术分析了在带状疱疹发病前和发病后较长时间内收集的癌症儿童血清。[3H]聚焦型和[35S]蛋氨酸标记水痘-带状疱疹病毒（VZV）特异性抗原的回收率在带状疱疹后立即增加数倍，在恢复期缓慢下降；然而，在两年内，两名患者的血清显示出VZV亚临床再激活的血清学证据。在免疫沉淀的电泳分离后，带状疱疹后的多肽谱与VZV的高效异种抗血清非常相似，并且含有至少16种成分，分子量从32到174,000不等。相比之下，在带状疱疹之前获得的血清很容易区分，因为它们很难沉淀两种主要的VZV糖蛋白（gp62和gp98）和几种非糖基化多肽。因此，带状疱疹的出现与以前无法检测到的vzv特异性蛋白抗体的出现有关。
Sera collected from children with cancer before and for extended periods after the onset of zoster were analyzed by radioimmune precipitation techniques. The percent recovery of both [3H]fucose- and [35S]methionine-labeled varicella-zoster virus (VZV)-specific antigens increased severalfold immediately after zoster and declined slowly during convalescence; however, within two years serum panels from two patients exhibited serologic evidence of subclinical reactivation of VZV. After electrophoretic fractionation of the immunoprecipitates, the polypeptide profile after zoster closely resembled that described for high titer xenoantisera to VZV and contained at least 16 constituents ranging in molecular weight from 32 to 174,000. In contrast, sera obtained before zoster were easily distinguished because they precipitated poorly, if at all, two major VZV glycoproteins (gp62 and gp98) and several nonglycosylated polypeptides. The emergence of zoster, therefore, was associated with the appearance of previously undetectable antibodies to VZV-specific proteins.