Morphine Can Inhibit the Growth of Breast Cancer MCF-7 Cells by Arresting the Cell Cycle and Inducing Apoptosis
Morphine Can Inhibit the Growth of Breast Cancer MCF-7 Cells by Arresting the Cell Cycle and Inducing Apoptosis
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吗啡通过阻滞细胞周期、诱导细胞凋亡抑制乳腺癌MCF-7细胞的生长
DOI：
10.1248/bpb.b17-00215
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发表时间：
2017-10-01
影响因子：
2
通讯作者：
Xie, Yubo
中科院分区：
文献类型：
--
作者：
Chen, Yanhua;Qin, Yi;Xie, Yubo
吗啡被广泛用于缓解晚期癌症患者的癌症疼痛。然而，在接受吗啡镇痛的乳腺癌患者中，吗啡是否可以抑制或促进癌症的进展仍不清楚。因此，我们使用吗啡和纳洛酮处理的体外模型来研究吗啡对乳腺癌细胞系MCF-7的影响。在MCF-7细胞中加入不同浓度的吗啡（0.01 ~ 10 μ m），分别于12、24、36、48、60、72小时后进行培养。MTT法检测细胞活力，流式细胞仪检测细胞周期和凋亡。此外，通过集落形成测定进行细胞增殖。本研究发现，吗啡（0.01 ~ 10 μ m）能显著降低MCF-7细胞的细胞活力、生长和集落形成率，这与细胞周期进程阻滞于G 0/G1和G2/M期及MCF-7细胞凋亡有一定关系。纳洛酮沿着吗啡不能逆转上述作用，提示吗啡对MCF-7乳腺癌细胞生长和增殖的抑制可能是其独立作用，与阿片受体无关。吗啡可通过阻滞细胞周期抑制MCF-7细胞生长，促进细胞凋亡。因此，吗啡可能无法促进接受吗啡镇痛的乳腺癌患者的癌症进展。
Morphine is widely used for relieving cancer pain in patients with advanced cancer. However, whether morphine can suppress or promote the progression of cancer in breast cancer patients receiving morphine analgesia remains unclear. Therefore, we used an in vitro model treated with morphine and naloxone to investigate the effects of morphine on breast cancer cell line MCF-7. MCF-7 cells were cultured with different concentrations (0.01 to 10,am) of morphine at 12th, 24th, 36th, 48th, 60th and 72nd hours. Then, cell viability was measured through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell cycle and apoptosis assays were detected by flow cytometry (FCM). In addition, cell proliferation was conducted by colony formation assay. In this study, we have found that morphine (0.01 to 10 mu m) could significantly reduce the cell vitality, growth and colony formation rate of MCF-7 cells, which has a certain relationship with cell cycle progression arrested at the G0/G1 and G2/M phase and MCF-7 cells apoptosis. Moreover, naloxone along with morphine could not reverse these effects, which indicates that the inhibition of MCF-7 breast cancer cell growth and proliferation by morphine could be its independent effect, not associated with opioid receptors. Morphine can inhibit cell growth by blocking the cell cycle and promote apoptosis in MCF-7 cells. Hence, morphine may be unable to promote the progression of cancer in breast cancer patients receiving morphine analgesia.