Surface-enhanced Raman Scattering (SERS) method and instrumentation for genomics and biomedical analysis
Surface-enhanced Raman Scattering (SERS) method and instrumentation for genomics and biomedical analysis
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用于基因组学和生物医学分析的表面增强拉曼散射 (SERS) 方法和仪器
DOI：
10.1002/(sici)1097-4555(199909)30:9
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发表时间：
1999
影响因子：
2.5
通讯作者：
M. Simon
中科院分区：
文献类型：
--
作者：
T. Vo‐Dinh;D. Stokes;G. Griffin;M. Volkan;U. Kim;M. Simon
介绍了一种用于生物医学和基因组学分析的表面增强拉曼散射（Sers）方法和仪器的发展。该技术使用基于Sers标记的DNA基因探针进行基因检测和DNA作图。该检测方法使用纳米结构的金属基底作为SERS活性平台。表面增强的拉曼基因（塞尔根）探针可用于通过与这些探针互补的DNA序列杂交来检测DNA靶。该探针不需要使用放射性标记，并且具有提供灵敏度和选择性的巨大潜力。先进的仪器系统设计的点源光谱测量和多光谱成像（MSI）的描述。MSI概念允许使用快速扫描固态设备（例如声光可调谐滤波器（AOTF））对视场中的二维杂交平台上的每个像素的整个Sers光谱进行重新编码。塞尔根的方法及其在生物医学诊断，高通量分析和DNA图谱和测序的应用的有用性进行了讨论。版权所有© 1999年约翰威利父子有限公司。
The development of a surface-enhanced Raman scattering (SERS) method and instrument for use in biomedical and genomics analysis is described. The technology uses DNA gene probes based on SERS labels for gene detection and DNA mapping. The detection method uses nanostructured metallic substrates as SERS-active platforms. The surface-enhanced Raman gene (SERGen) probes can be used to detect DNA targets via hybridization to DNA sequences complementary to these probes. The probes do not require the use of radioactive labels and have great potential to provide both sensitivity and selectivity. Advanced instrumental systems designed for point-source spectral measurements and for multi-spectral imaging (MSI) are described. The MSI concept allows recoding the entire SERS spectrum for every pixel on the two-dimensional hybridization platform in the field of view with the use of a rapid-scanning solid-state device, such as the acousto-optic tunable filter (AOTF). The usefulness of the SERGen approach and its applications in biomedical diagnostics, high-throughput analysis and DNA mapping and sequencing are discussed. Copyright © 1999 John Wiley & Sons, Ltd.