POSTSYNAPTIC DORSAL COLUMN PATHWAY OF THE RAT .1. ANATOMICAL STUDIES
POSTSYNAPTIC DORSAL COLUMN PATHWAY OF THE RAT .1. ANATOMICAL STUDIES
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DOI：
10.1152/jn.1984.51.2.260
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
MADSEN, AM
中科院分区：
文献类型：
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作者：
GIESLER, GJ;NAHIN, RL;MADSEN, AM
利用辣根过氧化物酶（HRP）的逆行转运检查脊髓背角向背柱核的投射。在其他动物中，这种投射被称为突触后背柱（PSDC）通路，可能与大鼠的伤害感觉有关。在颈段C6-8的同侧灰质内的交替切片中，向楔形核（CN）标记bbb350神经元注射HRP。CN注射后，[腰椎]L4-6神经元标记少于25个。将HRP局限于细核（GN）标记的bbb200个神经元内，在横跨L4-6的同侧背角下胶状物质的窄带内注射。C6-8标记细胞少于10个。通过横断[胸]T10、T8或C2的背柱，可以阻止注射GN后腰椎神经元的标记。通过这种注射标记的神经元完全在背柱内上升。CN注射后，C2背柱病变减少了颈髓中标记神经元的数量。90%。背柱和同侧背外侧索（DLF）的联合病变使C6-8中标记的细胞数量减少了约1%。98%。颈部增大的大部分标记神经元通过背柱投射到CN；颈向CN投影的一个小的次要组成部分似乎在DLF内上升。通过计数颈、腰椎增大区灰质中标记神经元及相应的DRG，比较PSDC神经元和DRG神经元向背柱核投射的相对大小。在4只动物中，PSDC神经元占投射到CN和apprx神经元的约38%。30%的细胞投射到GN。大鼠的PSDC投射能够为背柱核提供一个大的躯体组织输入。
The projection to the dorsal column nuclei that originates in the spinal cord dorsal horn was examined using the retrograde transport of horseradish peroxidase (HRP). This projection in other animals has been called the postsynaptic dorsal column (PSDC) pathway and might be involved in nociception in the rat. Small iontophoretic injections of HRP into the cuneate nucleus (CN) labeled > 350 neurons in alternate sections within the ipsilateral gray matter of segments [cervical] C6-8. Fewer than 25 neurons were labeled in [lumbar] L4-6 by injections into CN. Injections of HRP confined to the gracile nucleus (GN) labeled > 200 neurons within a narrow band extending across the ipsilateral dorsal horn subjacent to substantia gelatinosa of L4-6. Fewer than 10 cells were labeled in C6-8 by such injections. Labeling in lumbar neurons following injections into GN was prevented by transection of the dorsal columns at [thoracic] T10, T8 or C2. Neurons labeled by such injections ascend entirely within the dorsal columns. Lesions of the dorsal columns in C2 reduced the number of labeled neurons in the cervical cord following CN injections by .apprx. 90%. Combined lesions of the dorsal columns and ipsilateral dorsal lateral funiculus (DLF) reduced the number of cells labeled in C6-8 by .apprx. 98%. The majority of labeled neurons in the cervical enlargement project to CN via the dorsal columns; a small secondary component of the cervical projection to CN appears to ascend within the DLF. The relative sizes of the projections to the dorsal column nuclei from PSDC neurons and dorsal root ganglion cells (DRG) were compared by counting labeled neurons in the gray matter of the cervical and lumbar enlargements and the corresponding DRG. In the 4 animals so examined, PSDC neurons constituted > 38% of the neurons that projected to CN and .apprx. 30% of the cells that projected to GN. The PSDC projection of the rat is capable of providing a large somatotopically organized input to the dorsal column nuclei.