Structural Stability and Increase in Size Rationalize the Efficiency of Lipoplexes in Serum
Structural Stability and Increase in Size Rationalize the Efficiency of Lipoplexes in Serum
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DOI：
10.1021/la8033726
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发表时间：
2009-03-03
期刊：
影响因子：
3.9
通讯作者：
Caracciolo, Giulio
中科院分区：
文献类型：
--
作者：
Marchini, Cristina;Montani, Maura;Caracciolo, Giulio
我们研究了血清对DDAB-DOPE/DNA和DC-Chol-DOPE/DNA脂质体的纳米结构、尺寸、表面电位、DNA结合量和转染率的影响。在没有血清的情况下，脂质体的纳米结构和DNA结合能力决定了转染率。当加入血清时，发现所有脂质体制剂的转染率都提高了。我们发现，结构稳定性和血清大小的增加是调节脂质体效率的主要参数。通过外推，我们认为血清通过调节抗性脂质-DNA复合体的大小，可以控制脂丛内化的机制，进而控制其效率。
We have investigated the effect of serum on nanometric structure, size, surface potential, DNA-binding capacity, and transfection efficiency of DDAB-DOPE/DNA and DC-Chol-DOPE/DNA lipoplexes as a function of membrane charge density and cationic lipid DNA charge ratio. In the absence of serum, the nanometric structure and DNA binding capacity of lipoplexes determined the transfection efficiency. When serum was added, the transfection efficiency of all lipoplex formulations was found to increase. We identified structural stability and an increase in size in serum as major parameters regulating the efficiency of lipofection. By extrapolation, we propose that serum, regulating the size of resistant lipid-DNA complexes, can control the mechanism of internalization of lipoplexes and, in turn, their efficiency.