Strontium incorporation improves the bone-forming ability of scaffolds derived from porcine bone
Strontium incorporation improves the bone-forming ability of scaffolds derived from porcine bone
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DOI：
10.1016/j.colsurfb.2017.11.070
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发表时间：
2018-02-01
影响因子：
5.8
通讯作者：
Ruan, Changshun
中科院分区：
文献类型：
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作者：
Cheng, Delin;Liang, Qingfei;Ruan, Changshun
尽管异种骨支架在骨缺损修复方面显示出潜力，但其促进骨再生的能力有待进一步提高。锶是骨中一种重要的微量元素，对骨修复有良好的作用。以表面多孔的煅烧猪骨(CPB)为基材，通过SrCl2和聚己内酯(PCL)的连续包覆，直接制备了一种含锶支架(CPB/PCL/SR)。基本表征表明，PCL涂层在提高力学性能的同时，更重要的是抑制了锶的释放。此外，还详细研究了人MSCs在CPB、CPB/PCL和CPB/PCL/SR上的体外行为。增殖、成骨基因表达、碱性磷酸酶染色和碱性磷酸酶活性的综合结果表明，PCL涂层对CPB的骨修复能力有轻微的影响。相反，CPB/PCL/SR比CPB更好地支持MSCs的成骨分化，突出了锶的作用。体内实验证实，CPB/PCL/SR的新骨形成效果好于CPB和CPB/PCL。这些结果验证了掺锶提高CPB成骨能力的优越性，为骨缺损的修复提供了一种有前景的替代方法。(C)2017爱思唯尔B.V.保留所有权利。
Although heterogeneous bone scaffolds have shown potential in bone defect repair, their capability of aiding bone regeneration need to be further enhanced. Strontium, one important trace element in bone, has a well-known favorable effect on bone repair. Here a strontium containing scaffold (CPB/PCL/Sr) based on superficially porous calcined porcine bone (CPB) was obtained straightforwardly by sequential coating of SrCl2 and polycaprolactone (PCL). The basic characterization revealed that PCL coating could simultaneously improve the mechanical properties and, more importantly, restrain strontium release. Moreover, in vitro behaviors of human MSCs on CPB, CPB/PCL, and CPB/PCL/Sr were studied in detail. The comprehensive results of proliferation, osteogenic gene expression, ALP staining, and ALP activity demonstrated that PCL coating slightly impaired the bone repair potential of CPB. In contrast, CPB/PCL/Sr better supported the osteogenic differentiation of MSCs than CPB, highlighting the role of strontium. The in vivo test confirmed a better new bone formation of CPB/PCL/Sr than CPB and CPB/PCL. These results verified the superiority of incorporating strontium to improve the bone-forming ability of CPB, offering a promising alternative for bone defect repair. (C) 2017 Elsevier B.V. All rights reserved.