Proteochondroitin sulfate synthesis and chondrogenic expression.
Proteochondroitin sulfate synthesis and chondrogenic expression.
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硫酸蛋白软骨素合成和软骨形成表达。
DOI：
10.1016/0014-4827(74)90476-5
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发表时间：
1974
影响因子：
3.7
通讯作者：
P. Royal
中科院分区：
文献类型：
--
作者：
P. Goetinck;J. P. Pennypacker;P. Royal
胚胎肢芽细胞在软骨形成表达发生的条件下培养。在这些条件下合成的硫酸蛋白软骨素的定量和定性估计。在软骨形成表达发生的培养期间，观察到掺入硫酸蛋白软骨素的十倍增加。这种增加反映在1%琼脂糖层析上鉴定的特定级分的优先增加中。在软骨表达的任何证据明显之前，可以在肢芽中鉴定出低水平的这种色谱级分。我们假设这种优先增加是软骨特异性事件。
Embryonic limb bud cells were cultured under conditions in which chondrogenic expression takes place. Quantitative and qualitative estimates were made of the proteochondroitin sulfate synthesized under these conditions. A tenfold increase in incorporation into proteochondroitin sulfate is observed over the culture period during which chondrogenic expression takes place. This increase is reflected in a preferential increase of a particular fraction identified on 1% agarose chomatography. Low levels of this chromatographic fraction can be identified in limb buds before any evidence of cartilage expression is evident. We postulate that this preferential augmentation is a cartilage specific event.