Luminescence investigation of photosensitizer distribution in skin: correlation of singlet oxygen kinetics with the microarchitecture of the epidermis
Luminescence investigation of photosensitizer distribution in skin: correlation of singlet oxygen kinetics with the microarchitecture of the epidermis
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DOI：
10.1117/1.jbo.18.11.115001
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发表时间：
2013-11
影响因子：
3.5
通讯作者：
J. Schlothauer;Julian Falckenhayn;Tobias Perna;S. Hackbarth;B. Röder
中科院分区：
文献类型：
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作者：
J. Schlothauer;Julian Falckenhayn;Tobias Perna;S. Hackbarth;B. Röder
抽象的。这是第一项研究表明，局部应用的光敏剂的单线态氧动力学与皮肤的微结构一致，例如，裂缝和毛囊。动力学表明单线态氧与皮肤的化学相互作用，这允许区分残留的乳霜，例如，毛囊口，光敏剂渗入皮肤。我们展示了一个易于使用的光纤应用的可行性，提供了原位调查的机会，以及设置与聚焦光学高分辨率二维扫描的单线态氧发光动力学在皮肤样品。结果表明，时间分辨的单线态氧发光检测在组织中是一个理想的工具，用于医学治疗，诊断和评估单线态氧与生物环境的相互作用。
Abstract. This is the first study showing that singlet oxygen kinetics of topically applied photosensitizers coincides with the microarchitecture of skin, e.g., fissures and hair follicles. The kinetics indicate a chemical interaction of singlet oxygen with the skin, which allows differentiating between residual crème, e.g., in the follicular orifice, and photosensitizer penetrated into the skin. We show the feasibility of an easy-to-use fiber optic application providing the opportunity for in situ investigation, as well as a setup with focused optics for high-resolution two-dimensional scanning of singlet oxygen luminescence kinetics in skin samples. The results show that time-resolved singlet oxygen luminescence detection in tissue is a desirable tool for medical therapy, diagnostics, and evaluation of singlet oxygen interaction with biological environments.