Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation
Bulk and single-cell alternative splicing analyses reveal roles of TRA2B in myogenic differentiation
复制标题
DOI：
10.1111/cpr.13545
复制
发表时间：
2023-09-13
影响因子：
8.5
通讯作者：
Luo，Wen
中科院分区：
文献类型：
--
作者：
Chen，Genghua;Chen，Jiahui;Luo，Wen
Alternative splicing (AS) disruption has been linked to disorders of muscle development, as well as muscular atrophy. However, the precise changes in AS patterns that occur during myogenesis are not well understood. Here, we employed isoform long‐reads RNA‐seq (Iso‐seq) and single‐cell RNA‐seq (scRNA‐seq) to investigate the AS landscape during myogenesis. Our Iso‐seq data identified 61,146 full‐length isoforms representing 11,682 expressed genes, of which over 52% were novel. We identified 38,022 AS events, with most of these events altering coding sequences and exhibiting stage‐specific splicing patterns. We identified AS dynamics in different types of muscle cells through scRNA‐seq analysis, revealing genes essential for the contractile muscle system and cytoskeleton that undergo differential splicing across cell types. Gene‐splicing analysis demonstrated that AS acts as a regulator, independent of changes in overall gene expression. Two isoforms of splicing factorTRA2Bplay distinct roles in myogenic differentiation by triggering AS ofTGFBR2to regulate canonical TGF‐β signalling cascades differently. Our study provides a valuable transcriptome resource for myogenesis and reveals the complexity of AS and its regulation during myogenesis.
