DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans
DR2539 is a novel DtxR-like regulator of Mn/Fe ion homeostasis and antioxidant enzyme in Deinococcus radiodurans
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DR2539 是一种新型 DtxR 样调节剂，可调节耐辐射球菌中的 Mn/Fe 离子稳态和抗氧化酶
DOI：
10.1016/j.bbrc.2010.04.106
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发表时间：
2010-05-28
影响因子：
3.1
通讯作者：
Hua, Yuejin
中科院分区：
文献类型：
--
作者：
Chen, Huan;Wu, Rongrong;Hua, Yuejin
白喉毒素阻遏物（DtxR）家族的转录调节因子控制参与铁和锰摄取的基因的表达，铁和锰不仅是必需的营养物质，而且被认为是微生物细胞内氧化还原循环所必需的。我们从耐辐射球菌中鉴定出一种独特的DtxR同源物（DR2539），该同源物具有特殊的特性，以其对辐射和氧化剂的极端抗性而闻名。突变株dr2539对过氧化氢的抗性高于野生型菌株R1。胞内过氧化氢酶活性测定和半定量PCR分析表明，该DtxR是过氧化氢酶（katE）的负调节因子。实时荧光定量PCR、转录谱和电感耦合等离子体质谱分析表明，DtxR通过维持胞内Mn/Fe离子的稳态参与了抗氧化系统的调控。耐辐射的然而，与其他DtxR同源物不同，D.耐辐射菌是锰转运蛋白基因（dr2283）的负调控子，也是铁依赖转运蛋白基因（dr1219，drb0125）的正调控子。耐辐射的(C)2010年爱思唯尔公司All rights reserved.
Transcriptional regulators of the diphtheria toxin repressor (DtxR) family control the expression of genes involved in the uptake of iron and manganese, which is not only necessitous nutrients but also was suggested to be essential for intracellular redox cycling of microorganisms. We identified a unique DtxR homologue (DR2539) with special characteristics from Deinococcus radiodurans, which is known for its extreme resistance to radiation and oxidants. The dr2539 mutant showed higher resistance to hydrogen peroxide than the wild-type strain R1 Intracellular catalase activity assay and semiquantitative PCR analysis demonstrated that this DtxR is a negative regulator of catalase (katE). Furthermore, quantitative real-time PCR, global transcription profile and inductively coupled plasma-mass spectrometry analysis showed that the DtxR is involved in the regulation of antioxidant system by maintaining the intracellular Mn/Fe ion homeostasis of D. radiodurans. However, unlike the other DtxR homologues, the DtxR of D. radiodurans acts as a negative regulator of a Mn transporter gene (dr2283) and as a positive regulator of Fe-dependent transporter genes (dr1219, drb0125) in D. radiodurans. (C) 2010 Elsevier Inc. All rights reserved.