Molecular classes of heterogeneous nuclear RNA in sea urchin embryos.
Molecular classes of heterogeneous nuclear RNA in sea urchin embryos.
复制标题
海胆胚胎中异质核RNA的分子类别。
DOI：
10.1016/0022-2836(75)90203-x
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发表时间：
1975
影响因子：
5.6
通讯作者：
M. Nemer
中科院分区：
文献类型：
--
作者：
L. Dubroff;M. Nemer
使用寡胸苷酸 (oligo (dT))-纤维素和聚尿苷酸 (poly (U)) 玻璃纤维过滤器，通过两种不同的方法将海胆囊胚的异质核 RNA 分为三个分子类别。我们将这些分子类别命名为 α、β 和 γ HnRNA‡，它们具有明显不同的特性：(i) α HnRNA 不与寡 (dT)-纤维素结合，而是与聚 (U) 过滤器结合，并通过 15% 甲酰胺从聚 (U) 中洗脱。该类的平均值（在变性溶剂中）为 37 S，包含 25 个残基的寡腺苷酸片段（oligo (A) 25），并且缺乏较大的聚腺苷酸片段（poly (A)）。通过端基分析确定，寡聚 (A) 25 不存在于分子的 3' 末端 (ii) β HnRNA 通过寡聚 (dT)-纤维素或聚 (U) 过滤器结合，并通过 40% 甲酰胺从后者中洗脱。该类的平均值为 31 S，在 3' 末端包含约 175 个残基的聚 (A) 片段，但缺少寡 (A) 25。此外，α 和 β HnRNA 均包含长度约 12 个残基的腺苷酸片段（寡 (A) 12）。(iii) γ HnRNA 不与寡 (dT) 或聚 (U) 结合。它的平均值为 36 S，并且缺乏聚 (A) 175 和寡聚 (A 25)。此外，我们的证据支持以下结论：这三类并非人工衍生自 HnRNA，而是包含体内 HnRNA 分子群，其平均值约为 36 S。
Heterogeneous nuclear RNA of sea urchin blastulae has been fractionated into three molecular classes by two separate methods, using oligothymidylate (oligo (dT))-cellulose and polyuridylate (poly (U)) glass fiber filters. These molecular classes, which we have designated α, β and γ HnRNA‡, have distinctly different properties:(i) α HnRNA is not bound by oligo (dT)-cellulose, but is bound by poly (U) filters, and is eluted from the poly (U) by 15% formamide. This class has a mean value (in denaturing solvent) of 37 S, contains an oligoadenylate segment of 25 residues (oligo (A) 25) and lacks larger polyadenylate segments (poly (A)). The oligo (A) 25 is not present at the 3′ terminus of the molecule, as determined by end-group analysis (ii) β HnRNA is bound by either oligo (dT)-cellulose or poly (U) filters and is eluted from the latter by 40% formamide. This class has a mean value of 31 S, contains a poly (A) segment of approximately 175 residues at the 3′ terminus, but lacks oligo (A) 25. In addition, both α and β HnRNAs contain adenylate stretches of approximately 12 residues in length (oligo (A) 12).(iii) γ HnRNA is not bound by either oligo (dT) or poly (U). It displays a mean value of 36 S and lacks both poly (A) 175 and oligo (A 25). Furthermore, our evidence supports the conclusion that these three classes are not artificially derived from HnRNA, but comprise an in vivo population of HnRNA molecules whose mean value is approximately 36 S.