Beneficial effect of antioxidants in purified neurons derived from rat cortical culture
Beneficial effect of antioxidants in purified neurons derived from rat cortical culture
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DOI：
10.1016/j.brainres.2006.10.092
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发表时间：
2007-02
期刊：
影响因子：
2.9
通讯作者：
J. Sagara;K. Fujiwara;Y. Sakakura;Hideyo Sato;S. Bannai;N. Makino
中科院分区：
文献类型：
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作者：
J. Sagara;K. Fujiwara;Y. Sakakura;Hideyo Sato;S. Bannai;N. Makino
从胎鼠大脑获得的脑细胞悬液进行培养，5天后从残余细胞中分离神经元。将这些纯化的神经元重新接种在培养皿上，在培养24小时内开始死亡。24 h后，这些神经元的谷氨酸含量迅速下降至不足初始水平的十分之一。在α-生育酚（一种众所周知的抗氧化剂）的存在下，神经元存活至少3天，尽管谷胱甘肽含量仍然很低。丁基羟基茴香醚有类似的效果，但抗坏血酸和尿酸没有或很少的影响。血清素被认为具有抗氧化活性，使神经元存活3天。这些结果表明，与其他类型的细胞分离的神经元由于氧化应激而不能存活，否则氧化应激可能被涉及谷胱甘肽的机制中和，并且包括血清素在内的抗氧化剂对这些纯化的神经元具有有益作用。
Brain cell suspensions obtained from cerebrum of fetal rats were cultured and after 5 days neurons were separated from the residual cells. These purified neurons, which were replated on the dish, started to die within 24 h in culture. Glutathione content of these neurons decreased rapidly to less than one-tenth of the initial level after 24 h. In the presence of α-tocopherol, a well-known antioxidant, the neurons survived for at least 3 days, though glutathione content remained very low. Butylated hydroxyanisol had similar effect, but ascorbic acid and uric acid had no or very little effect. Serotonin, which is assumed to have an antioxidant activity, kept the neurons alive for 3 days. These results suggest that neurons separated from the other types of cells cannot survive due to the oxidative stress, which may otherwise be neutralized by a mechanism involving glutathione, and that antioxidants including serotonin has a beneficial effect on these purified neurons.