Relationship between surrounding tissue morphology and directional collective migration of the posterior lateral line primordium in zebrafish
Relationship between surrounding tissue morphology and directional collective migration of the posterior lateral line primordium in zebrafish
复制标题
斑马鱼后侧线原基周围组织形态与定向集体迁移的关系
DOI：
10.1111/gtc.12793
复制
发表时间：
2020
期刊：
影响因子：
2.1
通讯作者：
Matsui Takaaki
中科院分区：
文献类型：
--
作者：
Karaiwa Akari;Yamada Sohei;Yamamoto Hodaka;Wakasa Mizuho;Ishijima Hannosuke;Akiyama Ryutaro;Hosokawa Yoichiroh;Bessho Yasumasa;Matsui Takaaki
集体细胞迁移，其中细胞聚集并一起移动，是胚胎发育，伤口愈合和癌症转移的重要过程。趋化因子信号传导引导细胞组装到它们的目的地。在斑马鱼后侧线原基（PLLP）中，已经提出由肌肉先锋细胞（MP）和肌快纤维（MFF）分泌的趋化因子配体Cxcl 12 a，其沿水平中线沿着分布，与PLLP中的受体Cxcr 4 b结合，Cxcl 12 a-Cxcr 4 b信号传导引导PLLP沿水平中线沿着由前向后迁移。然而，周围组织如何影响PLLP迁移仍有待阐明。在这里，我们研究了PLLP和周围组织之间的关系，发现背侧和腹侧肌节之间的沟是由Sonic hedgehog（Shh）信号转导依赖的MP和MFF分化产生的，PLLP在这个沟中迁移。当Shh信号的瞬时抑制损害表达cxcl 12 a的MP/MFF的沟形成和分化时，PLLP的定向迁移受到严重干扰。此外，当分化的MP和MFF被飞秒激光照射消融时，沟槽仍然存在，PLLP迁移相对不受影响。这些结果表明，背侧和腹侧肌节之间的沟形成与PLLP的迁移行为。
Collective cell migration, in which cells assemble and move together, is an essential process in embryonic development, wound healing and cancer metastasis. Chemokine signaling guides cell assemblies to their destinations. In zebrafish posterior lateral line primordium (PLLP), a model system for collective cell migration, it has been proposed that the chemokine ligand Cxcl12a secreted from muscle pioneer cells (MPs) and muscle fast fibers (MFFs), which are distributed along with the horizontal midline, binds to the receptor Cxcr4b in PLLP and that Cxcl12a–Cxcr4b signaling guides the anterior‐to‐posterior migration of PLLP along the horizontal midline. However, how the surrounding tissues affect PLLP migration remains to be elucidated. Here, we investigated the relationship between the PLLP and the surrounding tissues and found that a furrow between the dorsal and ventral myotomes is generated by Sonic hedgehog (Shh) signaling‐dependent MP and MFF differentiation and that the PLLP migrates in this furrow. When transient inhibition of Shh signaling impaired both the furrow formation and differentiation ofcxcl12a‐expressing MPs/MFFs, directional PLLP migration was severely perturbed. Furthermore, when differentiated MPs and MFFs were ablated by femtosecond laser irradiations, the furrow remained and PLLP migration was relatively unaffected. These results suggest that the furrow formation between the dorsal and ventral myotomes is associated with the migratory behavior of PLLP.