Non-radial tortuous migration with cell polarity alterations of newly generated granule neurons in the neonatal rat dentate gyrus
Non-radial tortuous migration with cell polarity alterations of newly generated granule neurons in the neonatal rat dentate gyrus
复制标题
新生大鼠齿状回新生颗粒神经元的非径向曲折迁移与细胞极性改变
DOI：
10.1007/s00429-019-01971-0
复制
发表时间：
2019
影响因子：
3.1
通讯作者：
Seki T
中科院分区：
文献类型：
--
作者：
Namba T;Shinohara H;Seki T
为了建立功能性神经元回路，新生神经元通常在胚胎期从心室生发区迁移到其最终位置。然而，海马齿状回的大多数兴奋性神经元是出生后在远离侧脑室的肺门中产生的。然后，新生成的颗粒神经元必须迁移到周围的颗粒细胞层（GCL），这表明新生颗粒细胞可能通过独特的细胞机制进行迁移。在本研究中，我们结合逆转录病毒标记和延时成像分析来描述出生后产生的颗粒神经元的迁移行为。我们的结果表明，虽然一半新生成的神经元经历径向迁移，但其余神经元则参与更复杂的迁移模式，包括转向和转动运动，并伴有过程形成和细胞极性改变。这些数据揭示了颗粒神经元分布在整个 GCL 中以促进海马回路的机制的多样性，而这些机制以前未被认识到。
To establish functional neuronal circuits, newborn neurons generally migrate from the ventricular germinal zones to their final positions during embryonic periods. However, most excitatory neurons of the hippocampal dentate gyrus are born postnatally in the hilus, far from the lateral ventricle. Newly generated granule neurons must then migrate to the surrounding granule cell layer (GCL), which suggests that newborn granule cells may migrate by unique cellular mechanisms. In the present study, we describe the migratory behaviors of postnatally generated granule neurons using combined retroviral labeling and time-lapse imaging analysis. Our results show that whereas half of the newly generated neurons undergo radial migration, the remainder engages in more complex migratory patterns with veering and turning movements accompanied by process formation and cell polarity alterations. These data reveal a previously unappreciated diversity of mechanisms by which granule neurons distribute throughout the GCL to contribute to hippocampal circuitry.