The exceptional responsiveness of certain human myeloid leukemia cells to colony-stimulating activity.
The exceptional responsiveness of certain human myeloid leukemia cells to colony-stimulating activity.
复制标题
某些人髓性白血病细胞对集落刺激活性的特殊反应。
作者：
J. Brennan;J. Dipersio;C. Abboud;M. Lichtman
我们研究了急性髓系白血病（AML）患者的骨髓细胞对集落刺激活性（CSA）的体外反应性。在液体培养中，CSA刺激AML细胞快速和广泛生长。在没有CSA的情况下，大多数细胞死亡。CSA还能刺激AML细胞的克隆生长，其最低需要量为刺激正常骨髓CFU-C生长所需量的1/10 ~ 1/50。AML细胞的CSA从Sephacryl S-200柱上洗脱，洗脱液的表观分子量为45，000道尔顿。该馏分也产生正常人骨髓的最佳刺激。在缓解期，患者的骨髓细胞在液体培养中不生长，并产生正常数量的粒细胞和红细胞集落响应CSA和促红细胞生成素。AML细胞的延长培养导致细胞分化，这通过增殖能力降低以及形态学和组织化学变化来证明。这些研究表明，某些AML细胞对CSA（一种正常粒细胞生成的体外介体）有异常反应。
We have studied the marrow cells from a patient with acute myeloid leukemia (AML) for their responsiveness to colony-stimulating activity (CSA) in vitro. The AML cells were stimulated by CSA to rapid and extended growth in liquid culture. In the absence of CSA, the majority of cells died. CSA also stimulated the clonal growth of AML cells, and the minimum requirement for CSA was one-tenth to one-fiftieth that required to stimulate the growth of normal marrow CFU-C. CSA for AML cells was eluted from Sephacryl S-200 columns in fractions that represented an apparent molecular weight of 45,000 daltons. This fraction also produced optimal stimulation of normal human marrow. During remission, the patient's marrow cells did not grow in liquid culture and produced normal numbers of granulocytic and erythroid colonies in response to CSA and erythropoietin. Extended culture of the AML cells resulted in cell differentiation evidenced by decreasing proliferative capacity and by morphological and histochemical changes. These studies indicate that certain AML cells are extraordinarily responsive to CSA, an in vitro mediator of normal granulopoiesis.