Laboratory maintenance of Agrobacterium.
Laboratory maintenance of Agrobacterium.
复制标题
农杆菌的实验室维护。
DOI：
10.1002/9780471729259.mc03d01s24
复制
发表时间：
2012
影响因子：
--
通讯作者：
Fuqua,Clay
中科院分区：
文献类型：
--
作者：
Morton,EliseR;Fuqua,Clay
农杆菌是根瘤菌的植物近缘种。一些物种会引起植物疾病，如冠瘤和毛状根，尽管也有无毒物种。根癌农杆菌是研究最深入的物种，它会引起冠瘤，这是一种发生在各种植物上的肿瘤性疾病。毒力是由大的质粒指定的，在这种情况下是OFA。这种肿瘤被称为钛(肿瘤诱导)质粒。在致病过程中，致病农杆菌复制Ti质粒的一段片段并将其转移到植物中，在植物基因组中整合并表达导致病害症状的基因。根癌农杆菌作为一种植物基因工程工具被广泛应用，也是一种模式微生物，在宿主-微生物联合、水平基因转移、细胞-细胞通讯和生物膜形成方面得到了很好的研究。本单元描述实验室培养OFA的标准方案。Tumefaciens.Curr.普罗托克。微生物。24：3D.1.1-3D.1.16。©2012 John Wiley&Sons，Inc.
Agrobacteriumspecies are plant‐associated relatives of the rhizobia. Several species cause plant diseases such as crown gall and hairy root, although there are also avirulent species.A. tumefaciensis the most intensively studied species and causes crown gall, a neoplastic disease that occurs on a variety of plants. Virulence is specified by large plasmids, and in the case ofA. tumefaciensthis is called the Ti (tumor‐inducing) plasmid. During pathogenesis, virulent agrobacteria copy a segment of the Ti plasmid and transfer it to the plant, where it subsequently integrates into the plant genome and expresses genes that result in the disease symptoms.A. tumefacienshas been used extensively as a plant genetic engineering tool, and is also a model microorganism that has been well studied for host‐microbe associations, horizontal gene transfer, cell‐cell communication, and biofilm formation. This unit describes standard protocols for laboratory cultivation ofA. tumefaciens.Curr. Protoc. Microbiol. 24:3D.1.1‐3D.1.16. © 2012 by John Wiley & Sons, Inc.
影响因子：
3.2
作者：
Blanca-Ordonez, Helena;Oliva-Garcia, Juan J.;Nogales, Joaquina
通讯作者：
Nogales, Joaquina
根瘤菌共生质粒的repABC基本复制子的不相容性和分配位点。
影响因子：
2.6
作者：
Nora E. Soberón;Tatiana Venkova;M. A. Ramírez;J. Téllez;M. A. Cevallos
通讯作者：
M. A. Cevallos