Interaction between toxin crystals and vegetative insecticidal proteins of Bacillus thuringiensis in lepidopteran larvae
Interaction between toxin crystals and vegetative insecticidal proteins of Bacillus thuringiensis in lepidopteran larvae
复制标题
DOI：
10.1007/s10526-017-9828-6
复制
发表时间：
2017-10-01
期刊：
影响因子：
2.5
通讯作者：
Kaznowski, Adam
中科院分区：
文献类型：
--
作者：
Baranek, Jakub;Konecka, Edyta;Kaznowski, Adam
以苏云金杆菌晶体毒素为基础的杀虫剂是很好的生物植保产品。然而，有些毒素的活性范围很窄，或者昆虫已经产生了抗药性。我们测试了B晶体的杀虫活性。单独的苏云金杆菌MPU B9菌株和补充有Vip3Aa蛋白的苏云金杆菌MPU B9菌株对抗重要害虫：甜菜夜蛾（夜蛾科：夜蛾科）、苹果蠹蛾（Cydia pomonella L.）（鳞翅目：卷蛾科）和松毛虫Dendrolimus pini L.（鳞翅目：马勃科）。Cry毒素对D. pini对S. exigua和C. Pomonella比Vip3Aa.通过少量营养杀虫蛋白补充Cry毒素表现出协同效应，并显著增强了杀虫剂的毒性。结果表明Cry和Vip3Aa毒素混合物用于控制作物和森林昆虫害虫群体的效用。
Insecticides based on crystalline toxins of Bacillus thuringiensis are very good biological plant protection products. However, the spectrum of activity of some toxins is narrow or resistance among insects has been developed. We tested the insecticidal activity of crystals of the B. thuringiensis MPU B9 strain alone and supplemented with Vip3Aa proteins against important pests: Spodoptera exigua Hubner (Lepidoptera: Noctuidae), Cydia pomonella L. (Lepidoptera: Tortricidae) and Dendrolimus pini L. (Lepidoptera: Lasiocampidae). The Cry toxins were more active for D. pini but less active against S. exigua and C. pomonella than Vip3Aa. Supplementation of Cry toxins by small amounts of vegetative insecticidal proteins demonstrated synergistic effect and significantly enhanced the toxicity of the insecticide. The results indicate the utility of Cry and Vip3Aa toxins mixtures to control populations of crops and forests insect pests.