Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum
Molecular characterization of nosRZDFYLX genes coding for denitrifying nitrous oxide reductase of Bradyrhizobium japonicum
复制标题
日本慢生根瘤菌反硝化一氧化二氮还原酶编码 nosRZDFYLX 基因的分子特征
DOI：
10.1023/b:anto.0000020156.42470.db
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
E. Bedmar
中科院分区：
文献类型：
--
作者：
L. Velasco;S. Mesa;Chang;María J. Delgado;E. Bedmar
来自日本慢生根瘤菌 USDA110 菌株的呼吸一氧化二氮还原酶的 nosRZDFYLX 基因簇已被克隆并测序。检测到对应于nosR、nosZ、结构基因、nosD、nosF、nosY、nosL和nosX的7个蛋白质编码区。推导的氨基酸序列与各种来源的其他一氧化二氮还原酶表现出高度的相似性。 NosZ 蛋白包含用于蛋白输出的信号肽。携带 nosZ 或 nosR 突变的突变菌株在硝酸盐存在下微需氧培养时会积累一氧化二氮。 PnosZ-lacZ 融合体在菌株 USDA110 中的最大表达需要同时存在低水平氧气条件和硝酸盐。融合的微氧激活需要 FixLJ 和 FixK2。
The nosRZDFYLX gene cluster for the respiratory nitrous oxide reductase from Bradyrhizobium japonicum strain USDA110 has been cloned and sequenced. Seven protein coding regions corresponding to nosR, nosZ, the structural gene, nosD, nosF, nosY, nosL, and nosX were detected. The deduced amino acid sequence exhibited a high degree of similarity to other nitrous oxide reductases from various sources. The NosZ protein included a signal peptide for protein export. Mutant strains carrying either a nosZ or a nosR mutation accumulated nitrous oxide when cultured microaerobically in the presence of nitrate. Maximal expression of a PnosZ-lacZ fusion in strain USDA110 required simultaneously both low level oxygen conditions and the presence of nitrate. Microaerobic activation of the fusion required FixLJ and FixK2.
影响因子：
2.9
作者：
Rasmussen, T;Berks, BC;Thomson, AJ
通讯作者：
Thomson, AJ