Monoaminergic neuronal development is not affected in PACAP-gene-deficient mice
Monoaminergic neuronal development is not affected in PACAP-gene-deficient mice
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DOI：
10.1016/j.regpep.2004.08.034
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发表时间：
2005-03-15
影响因子：
--
通讯作者：
Shioda, SJ
中科院分区：
文献类型：
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作者：
Ogawa, T;Nakamachi, T;Shioda, SJ
腺苷酸环化酶激活多肽（PACAP）参与多种生理功能。体外研究表明，PACAP在神经系统发育中起重要作用，如神经细胞的增殖和分化。最近，据报道，缺乏PACAP的小鼠在出生后不久就表现出较高的死亡率，热适应受损，以及精神行为改变。由于单胺能神经系统与这些表型有关，并且关于这种肽在体外神经发育中的作用的数据很少，我们研究了缺乏PACAP的小鼠单胺能神经系统的早期发育[胚胎10.5天（E10.5）和12.5天（E12.5）]。缺乏PACAP的胎儿显示酪氨酸羟化酶（TH）和5-羟色胺（5-HT）的免疫反应性（IR）与野生型相似。我们观察了前脑[纹状体分化区（dz）和下丘脑dz]，中脑，后脑，神经嵴源性交感神经节原基的TH-IR。腹侧脊髓dz和肠在E10.5在PACAP无效和野生型中没有差异。在E12.5野生型和PACAP基因缺陷小鼠中，在几个脑区中未观察到5-HT和TH-IR的差异。包括脑干（中脑和脑桥）。因此，PACAP的耗竭不会影响早期发育中的单胺能神经系统。(C)2004 Elsevier B. V.保留所有权利。
Pituitary adenylate cyclase-activating polypeptide (PACAP) has been implicated in several physiological functions. Several lines of evidence from in vitro studies have shown that PACAP plays some important roles in development of nervous System such as neural proliferation and differentiation. Recently, mice lacking PACAP have been reported to show a higher mortality shortly after birth, impaired thermal adaptation, and altered psychomotor behaviors. In as much as monoaminergic nervous systems are implicated in these phenotypes and a quite few data have been reported on the role of this peptide in nervous development in vitro, we studied early development [embryonic days 10.5 (E10.5) and 12.5 (E12.5)] of monoaminergic nervous systems in mice lacking PACAP. The fetuses lacking PACAP showed immunoreactivities (IRs) for tyrosine hydroxylase (TH) and serotonin (5-HT) similarly to the wild type. We observed TH-IR in the forebrain [striatal differentiating zone (dz) and hypothalamic dz], midbrain, hindbrain, neural-crest-derived sympathetic ganglionic primordia. ventral spinal cord dz, and bowel at E10.5 in both PACAP null and wild type with no difference. At E12.5 in the wild-type- and PACAP-gene-deficient mice, no differences of 5-HT- and TH-IRs were observed in several brain regions. including brainstem (midbrain and pons). Thus, the depletion of PACAP does not affect monoaminergic nervous systems in the early development. (C) 2004 Elsevier B.V. All rights reserved.