THE TRK PROTOONCOGENE PRODUCT - A SIGNAL TRANSDUCING RECEPTOR FOR NERVE GROWTH-FACTOR
THE TRK PROTOONCOGENE PRODUCT - A SIGNAL TRANSDUCING RECEPTOR FOR NERVE GROWTH-FACTOR
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DOI：
10.1126/science.1850549
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发表时间：
1991-04-26
期刊：
影响因子：
56.9
通讯作者：
PARADA, LF
中科院分区：
文献类型：
--
作者：
KAPLAN, DR;HEMPSTEAD, BL;PARADA, LF
trk原癌基因编码一种140千道尔顿的跨膜蛋白酪氨酸激酶（p140 prototrk），仅在神经组织中表达。 神经生长因子（NGF）刺激神经细胞系和胚胎背根神经节中p140蛋白的磷酸化。 用I-125标记的NGF进行的亲和交联和平衡结合实验表明，p140 prototrk在培养细胞中特异性结合NGF，解离常数为10（-9）摩尔。 p140 prototrk作为NGF受体的鉴定表明该蛋白参与了NGF的主要信号转导机制。
The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein tyrosine kinase (p140prototrk) that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of p140prototrk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with I-125-labeled NGF indicate that p140prototrk binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of p140prototrk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF.