Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy.
Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy.
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DOI：
10.4049/jimmunol.162.3.1765
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发表时间：
1999-02
影响因子：
4.4
通讯作者：
Katarzyna D. Bieganowska;P. Höllsberg;G. Buckle;D. Lim;Tim F Greten;J. Schneck;J. Altman;S. Jacobson;S. Ledis;B. Hanchard;J. Chin;O. Morgan;P. Roth;D. Hafler
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文献类型：
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作者：
Katarzyna D. Bieganowska;P. Höllsberg;G. Buckle;D. Lim;Tim F Greten;J. Schneck;J. Altman;S. Jacobson;S. Ledis;B. Hanchard;J. Chin;O. Morgan;P. Roth;D. Hafler
人类T细胞嗜淋巴细胞病毒-I（HTLV-I）相关性脊髓病是一种缓慢进展的神经系统疾病，其特征是中枢神经系统中的炎性浸润，伴有HTLV-I反应性CD 8 + T细胞的克隆扩增。在携带HLA-A2等位基因的患者中，免疫应答主要针对Tax 11 -19肽。使用载有Tax 11 -19肽的MHC I类四聚体测定HTLV-I相关性脊髓病患者中HLA-A2/Tax 11 -19结合T细胞的频率、活化状态和TCR使用。发现循环Tax 11 -19反应性T细胞的频率非常高，接近1：10循环CD 8 + T细胞。结合HLA-A2/Tax 11 -19的T细胞由表达不同趋化因子受体和IL-2 R β链但不表达IL-2 R α链的异质群体组成。此外，Tax 11 -19反应性CD 8 + T细胞使用一种主要的TCR V β链识别HLA-A2/Tax 11 -19复合物。这些数据为HTLV-I相关性脊髓病患者中高频率的循环Tax 11 -19反应性CD 8 + T细胞提供了直接证据。
Human T cell lymphotropic virus-I (HTLV-I)-associated myelopathy is a slowly progressive neurologic disease characterized by inflammatory infiltrates in the central nervous system accompanied by clonal expansion of HTLV-I-reactive CD8+ T-cells. In patients carrying the HLA-A2 allele, the immune response is primarily directed to the Tax11-19 peptide. The frequency, activation state, and TCR usage of HLA-A2/Tax11-19 binding T cells in patients with HTLV-I-associated myelopathy was determined using MHC class I tetramers loaded with the Tax11-19 peptide. Circulating Tax11-19-reactive T cells were found at very high frequencies, approaching 1:10 circulating CD8+ T cells. T cells binding HLA-A2/Tax11-19 consisted of heterogeneous populations expressing different chemokine receptors and the IL-2R beta-chain but not the IL-2R alpha-chain. Additionally, Tax11-19-reactive CD8+ T cells used one predominant TCR Vbeta-chain for the recognition of the HLA-A2/Tax11-19 complex. These data provide direct evidence for high frequencies of circulating Tax11-19-reactive CD8+ T cells in patients with HTLV-I-associated myelopathy.