Single mRNA tracking in live cells.
Single mRNA tracking in live cells.
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DOI：
10.1016/s0076-6879(10)72003-6
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发表时间：
2010
影响因子：
--
通讯作者：
Singer, Robert H.
中科院分区：
文献类型：
--
作者：
Park, Hye Yoon;Buxbaum, Adina R.;Singer, Robert H.
mRNA的不对称分布是在不同细胞类型中观察到的普遍现象。mRNA的转录后运动和定位提供了将某些蛋白质靶向其功能的特定细胞质区域的重要机制。最近的技术进步使活细胞中单个mRNA分子的实时可视化成为可能。分析单个mRNA运动的研究揭示了复杂的RNA转运系统。本章概述了单粒子跟踪的一般方法和用于单个mRNA检测的一些方法。
Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.