Identification of RING-H2 Gene Candidates Related to Wood Formation in Poplar
Identification of RING-H2 Gene Candidates Related to Wood Formation in Poplar
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与杨树木材形成相关的 RING-H2 候选基因的鉴定
DOI：
10.3390/f10080698
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发表时间：
2019-08
期刊：
影响因子：
2.9
通讯作者：
Cheng Yuxiang
中科院分区：
文献类型：
--
作者：
Tong Guimin;Shen Hongmei;Cao Shenquan;Xu Wenjing;Ma Xujun;Cheng Yuxiang
RING-H2基因是RING类基因中最丰富的编码泛素连接酶E3的基因，参与多种生物学过程。RING-H2基因是否与木材形成有关还有待于在树木中鉴定。在本研究中，我们在大果杨中鉴定了288个RING-H2基因，并发现片段和串联重复事件有助于RING-H2基因的扩增。微阵列数据集（来自Affyestuary白杨基因组阵列）显示，249个RING-H2基因中有64个在茎木质部中高度或优先表达。根据AspWood RNAseq数据集，基因PtrRHH 21、33、48、69、88、93、94、121、141、166、175、192、208、214、250和257的转录水平在从膨胀木质部到木质化木质部的范围内显著增加，表明它们与木材形成相关。启动子分析表明，大多数优先在木质部表达的RING-H2基因具有SNBE、TERE、M46 RE、AC和SMRE顺式元件，这些元件参与次生细胞壁的生物合成和程序性细胞死亡。基于启动子GUS的分析结果表明，PtrRHH 94与转基因梧桐的木材形成相关。总之，根据多个基因表达分析，已经鉴定了数十个与木材形成相关的杨树RING-H2基因候选者。
RING-H2 genes, the most abundant RING-type genes encoding putative ubiquitin ligase E3, are involved in diverse biological processes. Whether RING-H2 genes are related to wood formation remains to be identified in trees. In this study, we identified 288 RING-H2 genes in Populus trichocarpa, and found that the segmental and tandem duplication events contributed to RING-H2 gene expansion. Microarray dataset (from Affymetrix poplar genome arrays) showed that 64 of the 249 RING-H2 genes were highly or preferentially expressed in stem xylem. According to the AspWood RNAseq dataset, the transcription levels of genes PtrRHH21, 33, 48, 69, 88, 93, 94, 121, 141, 166, 175, 192, 208, 214, 250 and 257 were significantly increased in the xylem ranging from the expanding xylem to the lignifying xylem, suggesting their association with wood formation. Promoter analyses revealed that most of the preferentially xylem-expressed RING-H2 genes possessed SNBE, TERE, M46RE, AC and SMRE cis-elements, which are involved in secondary cell wall biosynthesis and programmed cell death. Based on the promoter GUS-based analysis result, PtrRHH94 was indicated to be associated with wood formation in transgenic P. trichocarpa. Taken together, dozens of Populus RING-H2 gene candidates associated with wood formation have been identified based on multiple gene expression analyses.
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