2,3-butanediol production by Enterobacter aerogenes:: selection of the optimal conditions and application to food industry residues

2,3-butanediol production by Enterobacter aerogenes:: selection of the optimal conditions and application to food industry residues
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DOI:
10.1007/s004490000210
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发表时间:
2000-12-01
期刊:
BIOPROCESS ENGINEERING
影响因子:
--
通讯作者:
Canepa, P
Canepa, P
中科院分区:
其他
文献类型:
--
作者:
Perego, P;Converti, A;Canepa, P

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实验确定了产气肠杆菌通过三组合成葡萄糖溶液分批发酵生产2,3-丁二醇的最佳温度、pH和起始底物浓度。在变温条件下进行的试验结果表明,最佳温度为39 ℃,并用于估计发酵和热灭活的活化能(7.19和23.6 kJ mol(-1))和相关熵(-0.32和-0.27 kJ mol(-1)K-1)。在可变pH值下的批运行证明了6.0的最佳pH值,其结果也用于对控制导致丁二醇的代谢途径的反应做出合理的假设。不同的食品工业废物,即淀粉水解物,白糖蜜和脱色糖蜜,和乳清的可发酵性,最后检查。
Optimum values of temperature, pH, and starting substrate concentration are experimentally determined for 2,3-butanediol production by Enterobacter aerogenes through three set of batch fermentations of synthetic glucose solutions. The results of tests carried out at variable temperature show an optimum of 39 degreesC and are used to estimate, for both fermentation and thermal inactivation, the activation enthalpies (7.19 and 23.6 kJ mol(-1)) and the related entropies (-0.32 and -0.27 kJ mol(-1) K-1). An optimum pH value of 6.0 is evidenced from batch runs at variable pH, whose results are also used to make reasonable hypotheses on the reaction controlling the metabolic pathway which leads to butanediol. The fermentability of different food industry wastes, namely starch hydrolysate, both ra iv and decoloured molasses, and whey, is finally checked.