Graphene Hydrogel as a Porous Scaffold for Cartilage Regeneration
Graphene Hydrogel as a Porous Scaffold for Cartilage Regeneration
复制标题
石墨烯水凝胶作为软骨再生的多孔支架
DOI：
10.1021/acsami.2c11307
复制
发表时间：
2022-11-29
影响因子：
9.5
通讯作者：
Lu, Jiayu
中科院分区：
文献类型：
--
作者：
Lyu, Chengqi;Cheng, Chi;Lu, Jiayu
多孔支架已被广泛应用于软骨组织再生。然而，通常很难理解支架材料的精细层次结构如何影响再生过程。石墨烯材料是用于坚固和生物相容性多孔结构的通用构建块，使得能够研究组织再生的结构线索，否则难以确定。在这里，我们利用石墨烯水凝胶作为模型支架的结构稳定和可调的检查与软骨细胞的支架上的向内生长的基质重塑的多孔结构的影响。我们观察到加速但平衡的软骨重塑与软骨细胞向内生长到表面上具有开放孔结构的石墨烯支架中相关。重要的是，这种增强的重塑选择性地促进II型胶原原纤维的表达超过蛋白聚糖聚集蛋白聚糖，因此清楚地说明软骨细胞在迁移到支架中时保持稳定的表型，同时为软骨修复的支架设计提供新的见解。
Porous scaffolds have widely been exploited in cartilage tissue regeneration. However, it is often difficult to understand how the delicate hierarchical structure of the scaffold material affects the regeneration process. Graphene materials are versatile building blocks for robust and biocompatible porous structures, enabling investigation of structural cues on tissue regeneration otherwise challenging to ascertain. Here, we utilize a graphene hydrogel with stable and tunable structure as a model scaffold to examine the effect of porous structure on matrix remodeling associated with ingrowth of chondrocytes on scaffolds. We observe much-accelerated yet balanced cartilage remodeling correlating the ingrowth of chondrocytes into the graphene scaffold with an open pore structure on the surface. Importantly, such an enhanced remodeling selectively promotes the expression of collagen type II fibrils over proteoglycan aggrecan, hence clearly illustrating that chondrocytes maintain a stable phenotype when they migrate into the scaffold while offering new insights into scaffold design for cartilage repair.