WHOLE CELL RECORDING FROM NEURONS IN SLICES OF REPTILIAN AND MAMMALIAN CEREBRAL-CORTEX
WHOLE CELL RECORDING FROM NEURONS IN SLICES OF REPTILIAN AND MAMMALIAN CEREBRAL-CORTEX
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DOI：
10.1016/0165-0270(89)90131-3
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发表时间：
1989-12-01
影响因子：
3
通讯作者：
KRIEGSTEIN, AR
中科院分区：
文献类型：
--
作者：
BLANTON, MG;LOTURCO, JJ;KRIEGSTEIN, AR
我们描述了从海龟的大脑半球以及大鼠和海龟的大脑切片中获得稳定的全细胞记录的方法。在维持内源性突触回路的细胞中，通过电压和电流箝位可以研究中枢神经元的突触电流和膜特性。本文描述的方法适用于未经修改的解剖显微镜和记录室，以及标准厚度（400-500 μ m）的脑切片。
We describe methods for obtaining stable, whole-cell recordings from neurons in brain hemispheres from turtles and in brain slices from rats and turtles. Synaptic currents and membrane properties of central neurons can be studied in voltage and current clamp in cells maintained within their endogenous synaptic circuits. The methods described here are compatible with unmodified dissecting microscopes and recording chambers, and with brain slices of standard thickness (400-500 .mu.m).