Effect of several growth factors on canine flexor tendon fibroblast proliferation and collagen synthesis in vitro
Effect of several growth factors on canine flexor tendon fibroblast proliferation and collagen synthesis in vitro
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DOI：
10.1016/j.jhsa.2004.12.006
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发表时间：
2005-05-01
影响因子：
1.9
通讯作者：
Gelberman, RH
中科院分区：
文献类型：
--
作者：
Thomopoulos, S;Harwood, FL;Gelberman, RH
目的：生长因子可用于加速肌腱愈合的速度。然而，在体内使用之前，生长因子对肌腱细胞的影响需要很好地表征。本研究的目的是评价4种生长因子对肌腱内成纤维细胞增殖和体外胶原生成的影响。我们的第一个假设是血小板衍生生长因子BB （PDGF-BB）和碱性成纤维细胞生长因子（bFGF）会促进细胞增殖和胶原蛋白的产生。我们的第二个假设是PDGF-BB和bFGF结合会产生积极的影响。方法：用犬屈肌腱成纤维细胞体外检测生长因子PDGF-BB、bFGF、血管内皮生长因子（VEGF）和骨形态发生蛋白2 （BMP-2）的表达。评估单一因素（PDGF-BB、bFGF、VEGF或BMP-2）或多种因素（PDGF-BB和bFGF）对细胞增殖（胸苷结合）和胶原生成（脯氨酸结合）的影响。结果：结果支持我们的假设。PDGF-BB和bFGF均能显著促进细胞增殖。PDGF-BB和bFGF也显著增加了胶原蛋白的生成。VEGF和BMP-2对细胞增殖和胶原生成均无影响。PDGF-BB联合bFGF存在剂量效应。与单独使用PDGF-BB和bFGF相比，联合使用PDGF-BB和bFGF导致细胞增殖增加，但胶原生成没有变化。结论：单独应用生长因子PDGF-BB和bFGF可显著促进屈肌腱成纤维细胞增殖和基质合成。PDGF-BB和bFGF联合给药导致单因子增殖增加。
Purpose: Growth factor delivery may be used to accelerate the rate of tendon healing. Before in vivo use, however, the effects of growth factors on tendon cells need to be well characterized. The purpose of this study was to evaluate the effects of 4 growth factors on intrasynovial tendon fibroblast proliferation and collagen production in vitro. Our first hypothesis was that platelet-derived growth factor BB (PDGF-BB) and basic fibroblast growth factor (bFGF) would promote cell proliferation and collagen production. Our second hypothesis was that there would be a positive effect from the combination of PDGF-BB and bFGF.Methods:The growth factors PDGF-BB, bFGF, vascular endothelial growth factor (VEGF), and bone morphogenetic protein 2 (BMP-2) were evaluated in vitro with canine flexor tendon fibroblasts. The effects of single factors (PDGF-BB, bFGF, VEGF, or BMP-2) or a combination of factors (PDGF-BB and bFGF) on cell proliferation (ie, thymidine incorporation) and collagen production (ie, proline incorporation) were evaluated.Results: The results supported our hypotheses. Cell proliferation increased significantly with PDGF-BB and bFGF. Collagen production also increased significantly wit PDGF-BB and bFGF. Cell proliferation and collagen production were unchanged with VEGF and BMP-2. A dose-response effect was seen for PDGF-BB combined with bFGF. The combination of PDGF-BB and bFGF led to an increase in cell proliferation but not change in collagen production compared with each factor alone.Conclusions: The growth factors PDGF-BB and bFGF significantly increased flexor tendon fibroblasts proliferation and matrix synthesis when applied singly. Administration of PDGF-BB and bFGF combined led to increased proliferation to single factors.