Mechanism of early light signaling by the carboxy-terminal output module of Arabidopsis phytochrome B.
Mechanism of early light signaling by the carboxy-terminal output module of Arabidopsis phytochrome B.
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DOI：
10.1038/s41467-017-02062-6
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发表时间：
2017-12-04
影响因子：
16.6
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Qiu Y;Pasoreck EK;Reddy AK;Nagatani A;Ma W;Chory J;Chen M
植物光敏色素被认为通过N末端光敏模块介导光敏色素相互作用转录因子（PIF）的降解来传递光信号，而C末端模块（包括组氨酸激酶相关结构域（HKRD））不参与信号传导。在这里，我们表明，拟南芥光敏色素B（PHYB）的C-末端模块是足以介导的降解PIF 3特异性和激活光合基因在黑暗中。HKRD是PHYB同源和异源二聚化的二聚化结构域。D1040 V突变破坏了HKRD的二聚化以及C-末端模块与PIF 3之间的相互作用，消除了PHYB核积累、光体生物发生和PIF 3降解。相反，破坏PIF 3和PHYB的N-末端模块之间的相互作用对PIF 3降解几乎没有影响。总之，这项研究表明，C-末端模块的二聚体形式通过将PHYB靶向亚核光体并与PIF 3相互作用以触发其降解而发挥重要的信号作用。植物光敏色素介导PIF转录因子降解为荧光信号。在这里，与以前的模型相反，Qiu等人表明PIF 3的降解不需要PHYB的N-末端光敏模块，而是可以由C-末端输出模块介导。
Plant phytochromes are thought to transduce light signals by mediating the degradation of phytochrome-interacting transcription factors (PIFs) through the N-terminal photosensory module, while the C-terminal module, including a histidine kinase-related domain (HKRD), does not participate in signaling. Here we show that the C-terminal module of Arabidopsis phytochrome B (PHYB) is sufficient to mediate the degradation of PIF3 specifically and to activate photosynthetic genes in the dark. The HKRD is a dimerization domain for PHYB homo and heterodimerization. A D1040V mutation, which disrupts the dimerization of HKRD and the interaction between C-terminal module and PIF3, abrogates PHYB nuclear accumulation, photobody biogenesis, and PIF3 degradation. By contrast, disrupting the interaction between PIF3 and PHYB’s N-terminal module has little effect on PIF3 degradation. Together, this study demonstrates that the dimeric form of the C-terminal module plays important signaling roles by targeting PHYB to subnuclear photobodies and interacting with PIF3 to trigger its degradation. Plant phytochromes mediate the degradation of PIF transcription factors to transduce light signaling. Here, contrary to previous models, Qiu et al. show that degradation of PIF3 does not require the N-terminal photosensory module of PHYB but can instead be mediated by the C-terminal output module.
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影响因子：
5.6
作者：
Ashenberg, Orr;Keating, Amy E.;Laub, Michael T.
通讯作者：
Laub, Michael T.
影响因子：
4.5
作者：
Kikis, Elise A.;Oka, Yoshito;Hudson, Matthew E.;Nagatani, Akira;Quail, Peter H.
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影响因子：
11.6
作者：
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通讯作者：
Sharrock, Robert A.
DOI：
10.1073/pnas.1221738110
发表时间：
2013-01-22
影响因子：
11.1
作者：
Hu, Wei;Franklin, Keara A.;Lagarias, J. Clark
通讯作者：
Lagarias, J. Clark
DOI：
10.1073/pnas.89.11.5039
发表时间：
1992-06-01
影响因子：
11.1
作者：
CHERRY, JR;HONDRED, D;VIERSTRA, RD
通讯作者：
VIERSTRA, RD