Prevalence of endogenous CD34+ adipose stem cells predicts human fat graft retention in a xenograft model.
Prevalence of endogenous CD34+ adipose stem cells predicts human fat graft retention in a xenograft model.
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DOI：
10.1097/prs.0b013e31829fe5b1
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发表时间：
2013-10
影响因子：
3.6
通讯作者：
Rubin JP
中科院分区：
文献类型：
--
作者：
Philips BJ;Grahovac TL;Valentin JE;Chung CW;Bliley JM;Pfeifer ME;Roy SB;Dreifuss S;Kelmendi-Doko A;Kling RE;Ravuri SK;Marra KG;Donnenberg VS;Donnenberg AD;Rubin JP
脂肪移植是一种很有前途的软组织增强技术，尽管移植物保留是高度不可预测的，影响移植物存活的因素还没有很好地确定。由于其分化和生长因子释放的能力，脂肪源性干细胞可能在移植愈合中发挥关键作用。作者的目的是确定存在于人类脂肪中的脂肪源性干细胞的生物学特性是否与移植物体积保留的体内结果相关。使用标准化离心技术处理来自8名人类受试者的脂肪抽吸物，然后皮下注射到6周龄无胸腺裸鼠的侧腹。移植物的质量和体积进行了测量，并进行组织学评价，包括CD 31+染色的血管，移植后8周。通过多参数流式细胞术分析从每个受试者收获时分离的基质血管部分的表面标志物，并评估增殖、分化能力和常氧/缺氧血管内皮生长因子分泌。受试者间基质血管组分中CD 34+祖细胞的百分比差异很大，平均为21.3% ± 15%（均值± SD）。间质血管成分细胞的增殖率和成脂潜力表现出中度患者间差异。在小鼠异种移植物研究中，8周后的保留体积范围约为36%至68%，总体平均值为52% ± 11%。CD 34+祖细胞的间质血管分数百分比与高移植物保留之间存在强相关性（r = 0.78，斜率= 0.76，p < 0.05）。患者之间存在脂肪组织的固有生物学差异。特别是，基质血管组分中的CD 34+祖细胞浓度可能是用于预测人脂肪移植物保留的因素之一。（Plast。重建132：845，2013）。
Fat grafting is a promising technique for soft-tissue augmentation, although graft retention is highly unpredictable and factors that affect graft survival have not been well defined. Because of their capacity for differentiation and growth factor release, adipose-derived stem cells may have a key role in graft healing. The authors’ objective was to determine whether biological properties of adipose-derived stem cells present within human fat would correlate with in vivo outcomes of graft volume retention. Lipoaspirate from eight human subjects was processed using a standardized centrifugation technique and then injected subcutaneously into the flanks of 6-week-old athymic nude mice. Graft masses and volumes were measured, and histologic evaluation, including CD31+ staining for vessels, was performed 8 weeks after transplantation. Stromal vascular fraction isolated at the time of harvest from each subject was analyzed for surface markers by multi-parameter flow cytometry, and also assessed for proliferation, differentiation capacity, and normoxic/hypoxic vascular endothelial growth factor secretion. Wide variation in percentage of CD34+ progenitors within the stromal vascular fraction was noted among subjects and averaged 21.3 ± 15 percent (mean ± SD). Proliferation rates and adipogenic potential among stromal vascular fraction cells demonstrated moderate interpatient variability. In mouse xenograft studies, retention volumes ranged from approximately 36 to 68 percent after 8 weeks, with an overall average of 52 ± 11 percent. A strong correlation (r = 0.78, slope = 0.76, p < 0.05) existed between stromal vascular fraction percentage of CD34+ progenitors and high graft retention. Inherent biological differences in adipose tissue exist between patients. In particular, concentration of CD34+ progenitor cells within the stromal vascular fraction may be one of the factors used to predict human fat graft retention. (Plast. Reconstr. Surg. 132: 845, 2013.)