Distribution of human papillomavirus 58 and 52 E6/E7 variants in cervical neoplasia in Chinese women
Distribution of human papillomavirus 58 and 52 E6/E7 variants in cervical neoplasia in Chinese women
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DOI：
10.1016/j.ygyno.2010.08.032
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发表时间：
2010-12-01
影响因子：
4.7
通讯作者：
Xie, Xing
中科院分区：
文献类型：
--
作者：
Ding, Tian;Wang, Xinyu;Xie, Xing
目标.特定类型内HPV基因组变异可能与特定地理区域和特定人群中宫颈癌的发展相关。人乳头瘤病毒（HPV）58和52已被发现在包括中国妇女在内的亚洲妇女中相对流行。本研究旨在评估中国妇女中HPV 58和52变异体与宫颈癌及其前体的风险。共采集宫颈标本2021份。DNA提取和基因分型后，通过直接测序分析了总共298份（177份HPV 58-单阳性和121份HPV 52-单阳性）DNA样本的E6和E7序列变异。结果。共发现29种新的HPV 58和52变异株，其中HPV 58 E7中C632 T（T201）和G760 A（G63 S）变异株与肿瘤严重程度呈正相关（P趋势
Objectives. The specific intratype HPV genome variations may be associated with the development of cervical cancer in specific geographic regions and a given population. Human papillomavirus (HPV) 58 and 52 have been found to be relatively prevalent among Asian women including Chinese women. This study aimed to assess the risk of HPV 58 and 52 variants for cervical cancer and its precursors in Chinese women.Methods. A total of 2021cervical samples were collected. After DNA extraction and genotyping, a total of 298 (177 HPV58-single positive and 121 HPV52-single positive) DNA samples were analyzed for E6 and E7 sequence variations by direct sequencing.Results. A total of 29 new reported variations of HPV 58 and 52 were found. For HPV58, the presence of C632T (T201) and G760A (G63S) variants in E7 showed a positive trend of the association with the severity of neoplasia (P trend