Markedly decreased blood perfusion of pancreatic islets transplanted intraportally into the liver: disruption of islet integrity necessary for islet revascularization.
Markedly decreased blood perfusion of pancreatic islets transplanted intraportally into the liver: disruption of islet integrity necessary for islet revascularization.
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DOI：
10.2337/db10-0895
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发表时间：
2012-03
期刊：
影响因子：
7.7
通讯作者：
Carlsson PO
中科院分区：
文献类型：
--
作者：
Henriksnäs J;Lau J;Zang G;Berggren PO;Köhler M;Carlsson PO
实验研究表明门静脉内移植胰岛的血运重建率较低。本研究旨在首次量化肝内移植胰岛的血液灌注，并阐明该部位适当胰岛移植血运重建的必要因素。移植所有细胞中表达荧光蛋白的黄变色龙蛋白3.0胰岛。通过微球测定移植血灌注。使用在 Tie-2 启动子下表达绿色荧光蛋白的胰岛评估血管密度和供体血管在血运重建中的相对贡献。移植后 1 个月时，肝内胰岛的血液灌注平均仅为天然胰岛的 5%。然而，存在明显的异质性，即在未经事先培养的情况下移植的胰岛中血液灌注减少较少，而在许多情况下在完整性被破坏的胰岛中恢复。血管密度分析表明，扭曲的胰岛得到了很好的血运重建，而移植后 1 个月仍然完好的胰岛几乎没有血管。在新的胰岛脉管系统中观察到很少的供体内皮细胞。门静脉内移植的胰岛的血液灌注非常低，可能容易发生缺血并妨碍胰岛功能。由于供体内皮细胞在移植后不会扩张，因此受体血管发生血运重建必须破坏胰岛完整性。
Experimental studies indicate low revascularization of intraportally transplanted islets. This study aimed to quantify, for the first time, the blood perfusion of intrahepatically transplanted islets and elucidate necessary factors for proper islet graft revascularization at this site. Yellow chameleon protein 3.0 islets expressing fluorescent protein in all cells were transplanted. Graft blood perfusion was determined by microspheres. The vascular density and relative contribution of donor blood vessels in revascularization was evaluated using islets expressing green fluorescent protein under the Tie-2 promoter. Blood perfusion of intrahepatic islets was as a mean only 5% of that of native islets at 1-month posttransplantation. However, there was a marked heterogeneity where blood perfusion was less decreased in islets transplanted without prior culture and in many cases restored in islets with disrupted integrity. Analysis of vascular density showed that distorted islets were well revascularized, whereas islets still intact at 1-month posttransplantation were almost avascular. Few donor endothelial cells were observed in the new islet vasculature. The very low blood perfusion of intraportally transplanted islets is likely to predispose for ischemia and hamper islet function. Since donor endothelial cells do not expand posttransplantation, disruption of islet integrity is necessary for revascularization to occur by recipient blood vessels.
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影响因子：
7.7
作者：
Lee, Young;Ravazzola, Mariella;Unger, Roger H.
通讯作者：
Unger, Roger H.
影响因子：
8.2
作者：
Kampf, C;Lau, T;Carlsson, PO
通讯作者：
Carlsson, PO
影响因子：
7.7
作者：
Christoffersson G;Henriksnäs J;Johansson L;Rolny C;Ahlström H;Caballero-Corbalan J;Segersvärd R;Permert J;Korsgren O;Carlsson PO;Phillipson M
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Phillipson M
影响因子：
7.7
作者：
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通讯作者：
Pipeleers D
影响因子：
7.7
作者：
Brissova, M;Fowler, M;Powers, AC
通讯作者：
Powers, AC