Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle
Exercise rapidly increases expression of the monocarboxylate transporters MCT1 and MCT4 in rat muscle
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DOI：
10.1113/jphysiol.2004.073478
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发表时间：
2004-11-15
影响因子：
5.5
通讯作者：
Bonen, A
中科院分区：
文献类型：
--
作者：
Coles, L;Litt, J;Bonen, A
我们研究了单次运动对大鼠比目鱼肌（SOL）、红肌（RG）和白色腓肠肌（WG）中单羧酸转运蛋白MCT 1和MCT 4蛋白和mRNA表达的影响。肌肉样本分别在运动前2小时（21 m min（-1），15%等级）和运动后即刻，以及运动后5，10和24小时获得。在2 h的运动过程中，对照组（+60%）和对照组（+56%）的MCT 1蛋白表达量显著增加（P < 0.05）。MCT 1蛋白在运动后10 h增加最多，分别为RG（+157%）、WG（+193%）和SOL（+179%）（P < 0.05）。运动后24 h，MCT 1蛋白在WG组（+100%）和SOL组（+55%）仍呈上调（P < 0.05），而在RG组无明显上调。运动组运动后MCT 1 mRNA表达水平持续升高至运动后24 h（P < 0.05），而对照组运动后5 h和10 h MCT 1 mRNA表达水平短暂下降至运动前水平，运动后24 h再次升高至运动前水平（P < 0.05）。运动时，RG组（+41%）和SOL组（+98%）MCT 4蛋白表达增加（P > 0.05）。运动后10 h，运动组（+131%）和运动组（+323%）的MCT 4蛋白表达均达到峰值（P < 0.05）。运动后24 h，MCT 4蛋白表达仍明显上调（RG：+106%; SOL：+225%）（P < 0.05）。运动后10 h和24 h，运动组大鼠胃黏膜中MCT 4 mRNA水平分别为+132%和+55%（P < 0.05）。这些研究表明，MCT 1和4蛋白是瞬时上调由一个单一回合的运动，涉及转录后和转录机制。因此，MCT 1和MCT 4属于一类选择的代谢基因，其在运动刺激下非常快速地上调。
We examined the effect of a single exercise session on the protein and mRNA expression of the monocarboxylate transporters MCT1 and MCT4 in rat soleus (SOL), and red (RG) and white gastrocnemius (WG) muscles. Muscle samples were obtained at rest before 2 h of treadmill exercise (21 m min(-1), 15% grade) and immediately after exercise, as well as 5, 10 and 24 h after exercise. During the 2 h exercise bout, MCT1 proteins in RG (+60%) and WG (+56%) were increased (P < 0.05). MCT1 protein was further increased thereafter, with peak increments occurring 10h after exercise in RG (+157%), WG (+193%) and SOL (+179%) (P < 0.05). Twenty-four hours after exercise, MCT1 protein was still up-regulated in WG (+100%) and SOL (+55%) (P < 0.05), but not in RG. MCT1 mRNA was up-regulated during exercise in RG (+53%) and WG (+98%) and remained elevated until 24h post-exercise in RG (P < 0.05), but in WG, MCT1 mRNA decreased transiently to pre-exercise levels at 5 and 10h after exercise, before increasing again at 24h (+150%) (P 0.05). In contrast, during exercise, in RG (+41%) and SOL (+98%) MCT4 protein was increased (P > 0.05). Peak increases in MCT4 protein were observed 10 h after exercise in RG (+131%) and SOL (+323%) (P < 0.05). MCT4 protein was still up-regulated 24 h after exercise (RG: +106%; SOL +225%) (P < 0.05). MCT4 mRNA in RG was not increased until 10 (+132%) and 24 h after exercise (+55%) (P < 0.05). These studies have shown that MCT1 and 4 proteins are transiently up-regulated by a single bout of exercise, involving post-transcriptional and transcriptional mechanisms. Thus, MCT1 and MCT4 belong to a class of selected metabolic genes that are very rapidly up-regulated with an exercise stimulus.