Liquid Chromatographic Analysis of the Interaction between Amino Acids and Aromatic Surfaces Using Single-Wall Carbon Nanotubes
Liquid Chromatographic Analysis of the Interaction between Amino Acids and Aromatic Surfaces Using Single-Wall Carbon Nanotubes
复制标题
使用单壁碳纳米管对氨基酸和芳香族表面相互作用进行液相色谱分析
DOI：
10.1021/acs.langmuir.5b02500
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Atsushi Hirano
中科院分区：
文献类型：
--
作者：
Kazuki Iwashita;Kentaro Shiraki;Rieko Ishii;Takeshi Tanaka;Atsushi Hirano
蛋白质对固体表面具有非特异性吸附能力。尽管非特异性吸附能力通常被理解为与表面的疏水性或电荷密度有关，但在氨基酸水平上对蛋白质与聚芳族表面（例如最近已用于生物技术应用的碳纳米管）之间的相互作用知之甚少。在这项研究中，我们研究了蛋白质氨基酸和碳纳米管之间的相互作用，使用高效液相色谱法对二氧化硅基质涂覆单壁碳纳米管（SWCNTs）。在本研究中使用的氨基酸中，色氨酸、酪氨酸和苯丙氨酸显示出对基质的特殊亲和力。这些氨基酸的特性亲和力归因于它们与单壁碳纳米管的大聚芳族表面的独特相互作用。这些结果有助于理解和控制蛋白质吸附到芳香族表面。
Proteins have nonspecific adsorption capacities for solid surfaces. Although the nonspecific adsorption capacities are generally understood to be related to the hydrophobicity or charge density of the surfaces, little is known at the amino acid level about the interaction between proteins and polyaromatic surfaces such as carbon nanotubes, which have recently been used for biotechnology applications. In this study, we investigated the interaction between proteinogenic amino acids and carbon nanotubes using high-performance liquid chromatography on silica matrices coated by single-wall carbon nanotubes (SWCNTs). Among the amino acids used in this study, tryptophan, tyrosine, and phenylalanine showed exceptional affinity for the matrices. The characteristic affinities of these amino acids were ascribed to their unique interactions with the large polyaromatic surfaces of the SWCNTs. These results are useful for understanding and controlling protein adsorption onto aromatic surfaces.