The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo
The yeast Wsc1 cell surface sensor behaves like a nanospring in vivo
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DOI：
10.1038/nchembio.220
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发表时间：
2009-11-01
影响因子：
14.8
通讯作者：
Dufrene, Yves F.
中科院分区：
文献类型：
--
作者：
Dupres, Vincent;Alsteens, David;Dufrene, Yves F.
在这里，我们报告使用单分子原子力显微镜在体内测量的细胞表面传感器的机械行为。我们专注于酵母壁应力组件传感器WSC 1，质膜蛋白，被认为是作为一个刚性的探针的细胞壁状态。我们首先映射活酵母细胞上的单个组氨酸标记的传感器的分布，通过扫描细胞表面与原子力显微镜提示携带nitrilotriacetate基团。然后，我们表明，Wsc 1的行为就像一个线性的纳米弹簧，能够抵抗高机械力和细胞表面应力的反应。基因组pmt 4缺失和Wsc 1中甘氨酸片段的插入导致蛋白质弹簧特性的实质性改变，支持细胞外丝氨酸/苏氨酸富集区糖基化的重要作用。
Here we report on in vivo measurement of the mechanical behavior of a cell surface sensor using single-molecule atomic force microscopy. We focus on the yeast wall stress component sensor Wsc1, a plasma membrane protein that is thought to function as a rigid probe of the cell wall status. We first map the distribution of individual histidine-tagged sensors on living yeast cells by scanning the cell surface with atomic force microscopy tips carrying nitrilotriacetate groups. We then show that Wsc1 behaves like a linear nanospring that is capable of resisting high mechanical force and of responding to cell surface stress. Both a genomic pmt4 deletion and the insertion of a stretch of glycines in Wsc1 result in substantial alterations in protein spring properties, supporting the important role of glycosylation at the extracellular serine/threonine-rich region.