Phytase Production by Aspergillus oryzae in Solid-State Fermentation and its Applicability in Dephytinization of Wheat Ban
Phytase Production by Aspergillus oryzae in Solid-State Fermentation and its Applicability in Dephytinization of Wheat Ban
复制标题
米曲霉固态发酵生产植酸酶及其在小麦脱植酸中的应用
DOI：
10.1007/s12010-014-0974-3
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发表时间：
2014
影响因子：
3
通讯作者：
Bijender Singh
中科院分区：
文献类型：
--
作者：
Sapna;Bijender Singh
米曲霉SBS50在初始底物与水分比为1:2，水活度为0.95的条件下，在30°C的麦麸固态发酵（SSF） 96 h后分泌出高滴度的植酸酶。在麦麸中添加蔗糖和牛肉提取物时，植酸酶的产量增加。当底物中添加表面活性剂Triton X-100 （145 U/g DMB）时，酶的产量进一步提高。通过优化，植酸酶的产量提高了29倍。在优化的条件下，发酵液中植酸酶的分泌量比深层发酵（SmF）高9.3倍。中温霉菌除分泌植酸酶外，还分泌淀粉酶、纤维素酶、果胶酶和木聚糖酶。扫描电镜显示稻瘟病菌在麦麸上生长繁茂，孢子丰富。该酶使麦麸脱盐，同时释放无机磷酸盐。
Aspergillus oryzae SBS50 secreted a high titre of phytase in solid-state fermentation (SSF) using wheat bran at 30 °C after 96 h at the initial substrate to moisture ratio of 1:2 and a water activity of 0.95. The production of phytase increased when wheat bran was supplemented with sucrose and beef extract. Further enhancement in enzyme production was recorded when the substrate was supplemented with the surfactant Triton X-100 (145 U/g of DMB). An overall 29-fold improvement in phytase production was achieved owing to optimization. Under optimized conditions, the mould secreted 9.3-fold higher phytase in SSF as compared to submerged fermentation (SmF). The mesophilic mould also secreted amylase, cellulase (CMCase), pectinase and xylanase along with phytase in SSF. Scanning electron microscopy revealed luxuriant growth of A. oryzae on wheat bran with abundant spores. The enzyme dephytinized wheat bran with concomitant liberation of inorganic phosphate.