TOPICAL DELIVERY ENHANCEMENT WITH MULTILAMELLAR LIPOSOMES INTO PILOSEBACEOUS UNITS .1. INVITRO EVALUATION USING FLUORESCENT TECHNIQUES WITH THE HAMSTER EAR MODEL
TOPICAL DELIVERY ENHANCEMENT WITH MULTILAMELLAR LIPOSOMES INTO PILOSEBACEOUS UNITS .1. INVITRO EVALUATION USING FLUORESCENT TECHNIQUES WITH THE HAMSTER EAR MODEL
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DOI：
10.1111/1523-1747.ep12611886
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发表时间：
1992-07-01
影响因子：
6.5
通讯作者：
WEINER, N
中科院分区：
文献类型：
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作者：
LIEB, LM;RAMACHANDRAN, C;WEINER, N
使用两种荧光技术，定量荧光显微术（QFM）和刮擦法（其中使用荧光分光光度法分析处理的皮肤的各种组织层），发现了表明脂质体递送到雄性叙利亚仓鼠耳膜的毛囊皮脂腺单元中的证据。将整个耳朵固定在Franz扩散池上，并用40 μ l以下测试制剂处理24小时，每一种含有几乎等于100 μ g/ml的羧基荧光素（CF）：i）多层磷脂酰胆碱：胆固醇：磷脂酰丝氨酸脂质体; ii）HEPES缓冲液（pH，7.4）; iii）5%丙二醇; iv）10%乙醇; v）0.05%月桂基硫酸钠;和vi）用于形成脂质体的相同脂质的悬浮液，所述脂质未被加工以产生双层构型。局部应用基于脂质体的制剂导致CF在毛囊皮脂腺单位中的累积显著高于应用任何其他非脂质体制剂。用于确定CF沉积到皮脂腺中的两种分析方法之间存在极好的相关性。
Evidence suggesting liposomal delivery into the pilosebaceous unit of the male Syrian hamster ear membrane was found using two fluorescent techniques, quantitative fluorescence microscopy (QFM), and a scraping method where the various tissue strata of treated skin are analyzed using fluorescence spectrophotometry.Whole ears were mounted on Franz diffusion cells and treated for 24 h with 40-mu-l of the following test formulations, each containing almost-equal-to 100-mu-g/ml carboxyfluorescein (CF): i) multilamellar phosphatidylcholine: cholesterol: phosphatidylserine liposomes; ii) HEPES buffer (pH, 7.4); iii) 5% propylene glycol; iv) 10% ethanol; v) 0.05% sodium lauryl sulfate; and vi) a suspension of the same lipids used to form the liposomes that were not processed so as to produce a bilayer configuration. Topical application of the liposomally based formulation resulted in a significantly higher accumulation of CF in the pilosebaceous units than the application of any of the other non-liposomal formulations. There was excellent correlation between the two analytical methods used to determine CF deposition into the sebaceous glands.