Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy
Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy
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DOI：
10.1530/rep-10-0170
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发表时间：
2010-10-01
期刊：
影响因子：
3.8
通讯作者：
Johnson, Greg A.
中科院分区：
文献类型：
--
作者：
Bailey, Daniel W.;Dunlap, Kathrin A.;Johnson, Greg A.
猪的子宫内膜功能主要由孕激素和包括雌激素在内的胎盘因子调控。孕激素水平在整个妊娠期间都很高，以刺激和维持子宫上皮组织细胞的分泌，这是胚胎（胚胎及其胚胎外膜）生长、着床、胎盘和发育所必需的。本研究确定了长期孕酮对猪子宫内膜腔上皮（LE）、腺上皮（GE）和脉管系统发育和组织结构的影响。猪在发情期间（第12天）去卵巢，然后每天注射玉米油或黄体酮，连续28 d。延长黄体酮治疗导致子宫角的重量和长度增加，子宫内膜和子宫肌层的厚度增加。GE细胞增生肥大不明显，但LE细胞高度增高，提示分泌活性升高。尽管GE发育不足，孕酮支持增加子宫内膜血管生成与妊娠相当。黄体酮也支持LE和GE的顶端和基底外侧结构域的改变。双花凝集素结合和α (v)整合素在LE和GE的顶端表面下调。Claudin-4, α (2) β(1)整合素和vimentin在基底外侧表面增加，而occludin -1和-2，claudin-3和E-cadherin不受孕酮处理的影响，表明结构上具有良好的跨上皮粘附和紧密的连接复合物。综上所述，研究结果表明，黄体酮影响LE、GE、血管发育和组织结构，但在缺乏卵巢或胎盘因素的情况下，黄体酮对GE发育的支持程度与妊娠相当。此外，LE和血管发育对黄体酮的作用高度敏感。复制（2010）140 583-594
In pigs, endometrial functions are regulated primarily by progesterone and placental factors including estrogen. Progesterone levels are high throughout pregnancy to stimulate and maintain secretion of histotroph from uterine epithelia necessary for growth, implantation, placentation, and development of the conceptus (embryo and its extra-embryonic membranes). This study determined effects of long-term progesterone on development and histoarchitecture of endometrial luminal epithelium (LE), glandular epithelium (GE), and vasculature in pigs. Pigs were ovariectomized during diestrus (day 12), and then received daily injections of either corn oil or progesterone for 28 days. Prolonged progesterone treatment resulted in increased weight and length of the uterine horns, and thickness of the endometrium and myometrium. Hyperplasia and hypertrophy of GE were not evident, but LE cell height increased, suggesting elevated secretory activity. Although GE development was deficient, progesterone supported increased endometrial angiogenesis comparable to that of pregnancy. Progesterone also supported alterations to the apical and basolateral domains of LE and GE. Dolichos biflorus agglutinin lectin binding and alpha(v) integrin were downregulated at the apical surfaces of LE and GE. Claudin-4, alpha(2)beta(1) integrin, and vimentin were increased at basolateral surfaces, whereas occludins-1 and -2, claudin-3, and E-cadherin were unaffected by progesterone treatment indicating structurally competent trans-epithelial adhesion and tight junctional complexes. Collectively, the results suggest that progesterone affects LE, GE, and vascular development and histoarchitecture, but in the absence of ovarian or placental factors, it does not support development of GE comparable to pregnancy. Furthermore, LE and vascular development are highly responsive to the effects of progesterone. Reproduction (2010) 140 583-594