Ultra-low fouling photocrosslinked coatings for the selective capture of cells expressing CD44
Ultra-low fouling photocrosslinked coatings for the selective capture of cells expressing CD44
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用于选择性捕获表达 CD44 的细胞的超低污染光交联涂层
DOI：
10.1016/j.msec.2020.111630
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Thissen Helmut
中科院分区：
文献类型：
--
作者：
Yoshikawa Chiaki;Nakaji-Hirabayashi Tadashi;Nishijima Nanami;Nonsuwan Punnida;Toh Rou Jun;Kowalczyk Wioleta;Thissen Helmut
生物界面相互作用的有效控制在广泛的生物医学应用中引起了人们的极大兴趣，从细胞培养工具到生物传感器和植入式医疗设备。对于许多这样的应用，需要细胞和材料表面之间高度特异性的相互作用。对这些相互作用的复杂控制一方面需要减少或防止非特异性相互作用，另一方面需要显示可以被细胞外受体识别的高度特异性信号。我们最近开发了超低污垢涂料，可以使用 2-羟丙基丙烯酰胺和 N-二苯甲酮丙烯酰胺的光反应性共聚物一步涂覆。在这里，我们通过将可聚合肽单体掺入这些共聚物中扩展了这种方法。首次通过噬菌体展示鉴定QQGWF序列为CD44的结合基序后，合成了单体QQGWFGAGK(丙烯酰胺)和丙烯酰胺-GAGQQGWF。我们的结果表明，由于 CD44 表达的差异，在 HepG2 和 HEK293 细胞存在的情况下，使用 QQGWFGAGK（丙烯酰胺）单体制造的 UV 交联涂层可有效选择性结合 hMSC。我们的结果还表明，使用 BCA 蛋白结合测定以及 E 分析，肽改性涂层保留了其低生物污染特性。 24 小时内的大肠杆菌附着测定。我们的方法提供了传统整合素介导的表面选择性细胞结合的替代方案，并利用跨膜蛋白 CD44 在多种疾病中高表达的事实，为新的诊断应用打开了大门。
The effective control of biointerfacial interactions is of outstanding interest in a broad range of biomedical applications, ranging from cell culture tools to biosensors and implantable medical devices. For many of these applications, highly specific interactions between cells and material surfaces are desired. Sophisticated control over these interactions requires reducing or preventing non-specific interactions on the one hand and displaying highly specific signals that can be recognized by extracellular receptors on the other. We have recently developed ultra-low fouling coatings that can be applied in a single step using photoreactive copolymers of 2-hydroxypropyl acrylamide andN-benzophenone acrylamide. Here, we have expanded this approach by incorporating polymerizable peptide monomers into these copolymers. The monomers QQGWFGAGK(acrylamide) and acrylamide-GAGQQGWF were synthesized after identifying the QQGWF sequence as a binding motif for CD44 by phage display for the first time. Our results demonstrate that UV-crosslinked coatings fabricated using the QQGWFGAGK(acrylamide) monomer are effective at selectively binding hMSC in the presence of HepG2 and HEK293 cells due to the difference in CD44 expression. Our results also demonstrate that the peptide modified coatings retain their low biofouling character using a BCA protein binding assay as well as anE. colibacterial attachment assay over a 24 h period. Our approach provides an alternative to traditional integrin-mediated selective cell binding on surfaces and opens the door to new diagnostic applications, exploiting the fact that the transmembrane protein CD44 is highly expressed in multiple diseases.