CHEMICAL CHARACTERIZATION OF THE COMPONENT OF THE JELLY COAT FROM SEA-URCHIN EGGS RESPONSIBLE FOR INDUCTION OF THE ACROSOME REACTION
CHEMICAL CHARACTERIZATION OF THE COMPONENT OF THE JELLY COAT FROM SEA-URCHIN EGGS RESPONSIBLE FOR INDUCTION OF THE ACROSOME REACTION
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DOI：
10.1016/0012-1606(79)90080-0
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
LENNARZ, WJ
中科院分区：
文献类型：
--
作者：
SEGALL, GK;LENNARZ, WJ
海胆卵周围的果冻涂层是一种多组分的细胞外基质，可以诱导精子中的顶体反应。4个种的果冻皮毛。它们的化学成分十分相似，分别为斑点沙棘、紫球球藻、竹叶球藻和变色石斑鱼。唾液蛋白(约占胶衣质量的20%)和岩藻糖多糖(约占胶衣质量的80%)是胶衣的主要大分子成分。顶体反应的诱导能力仅存在于岩藻糖硫酸酯多糖中。顶体反应的诱导范围从高度物种特异性到非特异性。斑点沙门氏菌和德氏沙门氏菌的精子只与其同源的凝胶层发生顶体反应，而紫斑点毛藻的精子与同源凝胶层或杂色沙门氏菌的凝胶层反应同样好，但不能与斑点沙门氏菌的凝胶层反应。杂色乳杆菌精子的反应似乎相对非特异性。顶体反应的物种特异性诱导仅存在于岩藻糖硫酸盐多糖中，这表明这种多糖在不同物种中肯定存在结构差异。在一些物种中，受精似乎涉及到在果冻毛水平和精子-卵子受体水平上识别精子-卵子。
Jelly coat, a multicomponent extracellular matrix surrounding the sea urchin egg, induces the acrosome reaction in sperm. The jelly coats of the 4 spp. studied (Arbacia punctulata, Strongylocentrotus purpuratus, S. droebachiensis and Lytechinus variegatus) were very similar in chemical composition. A sialoprotein (approximately 20% of the mass of the jelly coat) and a fucose sulfate polysaccharide (approximately 80%) are the major macromolecular components of the jelly coat. The acrosome reaction inducing capacity resides solely in the fucose sulfate polysaccharide. Induction of the acrosome reaction ranges from highly species specific to nonspecific. A. punctulata and S. droebachiensis sperm are induced to undergo the acrosome reaction only with their homologous jelly coat, while S. purpuratus sperm react equally well with homologous or L. variegatus jelly coat, but not with A. punctulata jelly coat. L. variegatus sperm seem relatively nonspecific in response. Species-specific induction of the acrosome reaction resides solely in the fucose sulfate polysaccharide, suggesting that there must be structural differences in this polysaccharide in the various species. In some species, fertilization appears to involve sperm-egg recognition at the level of the jelly coat and at the level of sperm-egg receptors.