Inhibition of surface membrane maturation in schistosomula of Schistosoma mansoni.
Inhibition of surface membrane maturation in schistosomula of Schistosoma mansoni.
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DOI：
10.1073/pnas.85.11.3825
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发表时间：
1988-06
影响因子：
11.1
通讯作者：
P. Wiest;A. Tartakoff;M. Aikawa;A. Mahmoud
中科院分区：
文献类型：
--
作者：
P. Wiest;A. Tartakoff;M. Aikawa;A. Mahmoud
曼氏血吸虫（Schistosoma mansoni）多细胞寄生虫的表面膜在尾蚴转化为童虫的过程中发生了根本性的重组。目前的研究调查的因素参与成熟的表面从三层到多层膜。当成熟诱导嘌呤霉素（900 μ M）的存在下，收购的多层膜表面和荧光素共轭Con A的寄生虫是类似的控制寄生虫。同样，虽然用莫能菌素（0.1 μ M）处理3小时的生物体在皮层下细胞的核周细胞质中显示出大的空泡，但表面膜变得多层。与此相反，微管活性药物干扰成熟：表面仍然主要是三层和有机体的百分比结合Con A到其表面显着减少。此外，在秋水仙素处理的寄生虫的被膜下细胞中发现了多层体的大量积累，而在对照中则很少看到。秋水仙素处理的童虫未能成熟成蠕虫注入小鼠后，像尾蚴，他们是水的耐受性。因此，我们的结论是，组成的组件，构成的童虫表面预先存在于尾蚴，并建议他们被运输到表面的微管的帮助下之前，被存储在多层机构。获得的多层膜可能是必要的寄生虫在体内和体外的生存。
The surface membrane of the multicellular parasite Schistosoma mansoni is radically reorganized during the transformation of cercariae into schistosomula. The current study investigates factors involved in maturation of the surface from a trilaminate to a multilaminate membrane. When maturation was induced in the presence of puromycin (900 microM), the acquisition of a multilaminate surface and stainability with fluorescein-conjugated Con A were similar to that of control parasites. Similarly, although organisms treated with monensin (0.1 microM) for 3 hr showed large vacuoles in the perinuclear cytoplasm of the subtegumental cells, the surface membrane became multilaminate. In contrast, microtubule-active drugs interfered with maturation: the surface remained largely trilaminate and the percentage of organisms binding Con A to their surface was significantly reduced. Furthermore, large accumulations of multilaminate bodies were found in the subtegumental cells of colchicine-treated parasites, whereas few were seen in the controls. Colchicine-treated schistosomula failed to mature to adult worms upon injection into mice and, like cercariae, they were water tolerant. We therefore conclude that the components that constitute the schistosomula surface preexist in cercariae and suggest that they are stored in multilaminate bodies before being transported to the surface with the help of microtubules. The acquisition of the multilaminate membrane may be essential for survival of the parasites in vivo and in vitro.