The use of electron beam lithographic graft-polymerization on thermoresponsive polymers for regulating the directionality of cell attachment and detachment
The use of electron beam lithographic graft-polymerization on thermoresponsive polymers for regulating the directionality of cell attachment and detachment
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DOI：
10.1016/j.biomaterials.2008.12.058
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发表时间：
2009-04-01
期刊：
影响因子：
14
通讯作者：
Kitamori, Takehiko
中科院分区：
文献类型：
--
作者：
Idota, Naokazu;Tsukahara, Takahiko;Kitamori, Takehiko
通过直接接枝聚合，已经开发了一种用于纳米图案化细胞培养基底的简单方法，该方法使用电子束（EB）光刻系统，不需要光掩模或EB敏感抗蚀剂。将N-异丙基丙烯酰胺（IPAAm）通过电子束曝光直接接枝到亲水性聚丙烯酰胺（PAAm）接枝的玻璃表面。通过改变电子束的照射面积，可以控制表面接枝聚合物的尺寸，并在表面上形成线宽为200 nm的最小条纹图案。在条纹图案化的表面上，在较低临界溶解温度（LCST）以上，细胞最初粘附并以沿着图案方向的取向扩散。贴壁细胞的伸展角和伸长的大小取决于接枝PIPAAm的图案间隔。当培养温度低于LCST时，培养的细胞从表面脱落，沿图案方向沿着收缩，有时折叠并与条纹图案平行。这种图案化的细胞回收技术可用于构建在特定方向上具有有效收缩/松弛的肌细胞片和球形3D细胞结构，并应用于组织工程和微流体细胞装置。(C)2009爱思唯尔有限公司保留所有权利。
A simple process for nano-patterned cell culture substrates by direct graft-polymerization has been developed using an electron beam (EB) lithography system requiring no photo-masks or EB-sensitive resists. The compound N-isopropylacrylamide (IPAAm) was locally polymerized and grafted directly by EB lithographic exposure onto hydrophilic polyacrylamide (PAAm)-grafted glass surfaces. The size of the surface grafted polymers was controlled by varying the area of EB dose, and a minimal stripe pattern with a 200 nm line-width could be fabricated onto the surface. On the stripe-patterned surfaces, above the lower critical solution temperature (LCST), the cells initially adhered and spread with an orientation along the pattern direction. The magnitude of the spreading angle and elongation of adhered cells depended on the pattern intervals of the grafted PIPAAm. When culture temperature was lower than the LCST, cultured cells detached from the surfaces with strong shrinkage along the pattern direction, and sometimes folded and became parallel with the stripe pattern. This patterned cell recovery technique may be useful for the construction of muscle cell sheets with efficient shrinkage/relaxation in a specific direction and spheroidal 3D cell structures, with application to tissue engineering and microfluidic cellular devices. (C) 2009 Elsevier Ltd. All rights reserved.