Quantification of doripenem in human plasma and peritoneal fluid by high-performance liquid chromatography with ultraviolet detection
Quantification of doripenem in human plasma and peritoneal fluid by high-performance liquid chromatography with ultraviolet detection
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DOI：
10.1016/j.jchromb.2008.03.001
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发表时间：
2008-05-01
影响因子：
3
通讯作者：
Sueda, Taijiro
中科院分区：
文献类型：
--
作者：
Ikeda, Kayo;Ikawa, Kazuro;Sueda, Taijiro
建立了一种包括超滤去除血浆和腹腔液蛋白的简便、快速的高效液相色谱方法，用于测定人血浆和腹腔液中的多利培南浓度。血浆或腹腔液与1M3-吗啉丙磺酸缓冲液(pH 7.0)(1：1)立即混合以稳定多利培南。在300 nm波长处测定多利培南和内标的紫外吸光度。多利培南在人血浆和腹腔液中的线性范围为0.05~100微克/毫升。对于血浆，日内和日间精密度均小于3.41%(CV)，准确度在97.4%~101.7%之间。腹腔液的日内和日间精密度均小于2.98%(CV)，准确度在94.4%~103.9%之间。血浆和腹腔液的检出限均为0.02微克/毫升。该方法已用于血浆和腹腔液中多利培南的药物监测。(C)2008爱思唯尔B.V.保留所有权利。
A simple and rapid HPLC method that includes ultrafiltration to remove plasma and peritoneal fluid protein was developed to determine doripenem concentrations in human plasma and peritoneal fluid. Doripenem was stabilized by immediate mixing of the plasma or peritoneal fluid with 1 M 3-morpholinopropanesulfonic acid buffer (pH 7.0) (1: 1). Doripenem and an internal standard were detected by measuring their ultraviolet absorbance at 300 nm. The calibration curves for doripenem in human plasma and peritoneal fluid were linear from 0.05 to 100 mu g/mL. For plasma, both the intra- and the inter-day precision were less than 3.41% (CV), and the accuracy was between 97.4 and 101.7% above 0.05 mu g/mL. For peritoneal fluid, the intra- and the interday precision were less than 2.98% (CV), and the accuracy was between 94.4 and 103.9% above 0.05 mu g/mL. The limit of detection was 0.02 mu g/mL in both plasma and peritoneal fluid. The assay has been applied to the therapeutic drug monitoring of doripenem in both plasma and peritoneal fluid. (C) 2008 Elsevier B.V. All rights reserved.