Methane-rich saline alleviates cerulein-induced acute pancreatitis by inhibiting inflammatory response, oxidative stress and pancreatic apoptosis in mice
Methane-rich saline alleviates cerulein-induced acute pancreatitis by inhibiting inflammatory response, oxidative stress and pancreatic apoptosis in mice
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富含甲烷的盐水通过抑制小鼠炎症反应、氧化应激和胰腺细胞凋亡来缓解雨蛙素诱导的急性胰腺炎
DOI：
10.1016/j.intimp.2017.07.022
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发表时间：
2017-10-01
影响因子：
5.6
通讯作者：
Deng, Xiaoming
中科院分区：
文献类型：
--
作者：
Xie, Qun;Fei, Miaomiao;Deng, Xiaoming
背景：急性胰腺炎(AP)是一种潜在威胁生命的胃肠道疾病，涉及消化酶的细胞内激活和胰腺腺泡细胞的损伤。目的：探讨富含甲烷的生理盐水(MS)对急性胰腺炎的调节作用。方法：对雨蛙素诱发的急性胰腺炎小鼠给予不同剂量生理盐水(16ml/kg)。检测大鼠血清淀粉酶、脂肪酶活性及胰腺组织病理学改变。采用双抗体夹心法检测血清细胞因子肿瘤坏死因子α、白介素6、干扰素-γ和白介素10。用实时荧光定量聚合酶链式反应检测胰腺组织中这些炎性细胞因子的mRNA水平。用商品试剂盒检测髓过氧化物酶(MPO)和超氧化物歧化酶(SOD)。结果：MS治疗可逆转天青素诱导的急性胰腺炎小鼠血清淀粉酶、脂肪酶水平升高，减轻胰腺病理损害，降低肿瘤坏死因子α、IL-6、干扰素-γ和IL-10的表达。此外，MS还下调了胰腺MPO的表达，上调了SOD的表达，表明MS对AP具有抗氧化作用。结论：MS对天青素诱导的急性胰腺炎具有抗炎、抗氧化和抗细胞凋亡作用，有望成为临床治疗胰腺炎的有效药物。
Background: Acute pancreatitis (AP) is a potentially life-threatening gastrointestinal disease involving intracellular activation of digestive enzymes and pancreatic acinar cell injury. The present study was performed to investigate whether methane-rich saline (MS) was involved in the regulation of AP.Methods: MS (16 ml/kg) was administered at different dosing frequencies on mice with cerulein-induced AP. Serum amylase, lipase and histopathological changes in the pancreas tissue were measured. Serum cytokine TNF alpha, IL-6, IFN gamma and IL-10 were detected by ELISA. The mRNA levels of these inflammatory cytokines in the pancreas were detected by real time-PCR. Myeloperoxidase (MPO) and superoxide dismutase (SOD) were determined using commercial kits. Apoptosis was assessed by immunohistochemistry and Western blot.Results: MS treatment reversed the increased serum level of amylase and lipase, alleviated the pathological damage in the pancreas, and decreased the expression of TNF alpha, IL-6, IFN gamma and IL-10 in cerulean-induced AP mice. In addition, MPO was down-regulated and SOD was up-regulated in the MS treated pancreas, indicating that MS had an anti-oxidant effect against AP. Furthermore, MS protected pancreatic cells against cerulean induced apoptosis and abolished cleaved caspase-3.Conclusion: MS exerted anti-inflammatory, anti-oxidant and anti-apoptotic effects on cerulein-induced AP in mice and may proved to be a promising therapeutic agent for the clinical treatment of pancreatitis.