Determination of hydroxyproline in plasma and tissue using electrospray mass spectrometry
Determination of hydroxyproline in plasma and tissue using electrospray mass spectrometry
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DOI：
10.1016/s0731-7085(03)00359-5
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发表时间：
2003-12-04
影响因子：
3.4
通讯作者：
Hallak, H
中科院分区：
文献类型：
--
作者：
Kindt, E;Gueneva-Boucheva, K;Hallak, H
建立了一种简便、特异性强的测定生物样品中羟脯氨酸的方法。该方法可用于监测与胶原降解相关的病理状况，以及筛选治疗药物的疗效。通过水解制备组织或血浆样品，并使用加标校准曲线和LC/MS/MS测定其羟脯氨酸含量。使用API飞行时间（TOF）LC/MS评价方法的专属性，以暴露潜在干扰。本文提出的方法具有选择性强、简便、精密度高（<10% R.S.D.）方法的线性范围为0.010-10 μg/ml。© 2003 Elsevier B. V.保留所有权利。
A simple and highly specific method that was developed for the determination of hydroxyproline in biological samples is described. This method could potentially be used for monitoring pathological conditions related to collagen degradation, as well as for screening remedial pharmaceuticals for efficacy. Tissue or plasma samples were prepared by hydrolysis and their hydroxyproline content was determined using spiked calibration curves and LC/MS/MS. Specificity of the method was evaluated using an API Time-Of-Flight (TOF) LC/MS to expose potential interferences. The method proposed here appears to be selective, convenient, precise ( < 10% R.S.D.), accurate ( < 10% RE), and sensitive over a linear range of 0.010-10 μg/ml. © 2003 Elsevier B.V. All rights reserved.