Identification of water-deficit responsive genes in maritime pine (Pinus pinaster Ait.) roots
Identification of water-deficit responsive genes in maritime pine (Pinus pinaster Ait.) roots
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DOI：
10.1023/a:1021168811590
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发表时间：
2003-01-01
影响因子：
5.1
通讯作者：
Plomion, C
中科院分区：
文献类型：
--
作者：
Dubos, C;Plomion, C
根系对土壤环境因素的适应对于海松非常重要，海松是法国用于重新造林的主要针叶树种。该物种栖息地的气候变化多样，从冬季的洪水到夏季的干旱。目前还没有研究关注根系的形态、生理或分子变异以使其生长适应这样的环境。我们制定了一种策略，在根组织中分离干旱响应基因，以确定树木为应对干旱（影响木材生产力的主要问题）而进化的分子机制，并利用这些信息来提高干旱胁迫耐受性。为了方便进入根系，幼苗在水培溶液中生长。使用聚乙二醇作为渗透剂来诱导缺水。使用 cDNA-AFLP 技术，我们筛选了 2500 多个转录衍生片段，其中 33 个 (1.2%) 在非应激和应激培养基之间显示出存在/不存在的明显差异。然后通过反向北方分析这些转录本的相对丰度。这 33 个基因中只有两个在两种技术之间表现出显着相反的行为。根部缺水响应基因的鉴定和表征提供了对海松干旱胁迫响应中涉及的基因表达和调控模式的生理学理解。
Root adaptation to soil environmental factors is very important to maritime pine, the main conifer species used for reforestation in France. The range of climates in the sites where this species is established varies from flooded in winter to drought-prone in summer. No studies have yet focused on the morphological, physiological or molecular variability of the root system to adapt its growth to such an environment. We developed a strategy to isolate drought-responsive genes in the root tissue in order to identify the molecular mechanisms that trees have evolved to cope with drought (the main problem affecting wood productivity), and to exploit this information to improve drought stress tolerance. In order to provide easy access to the root system, seedlings were raised in hydroponic solution. Polyethylene glycol was used as an osmoticum to induce water deficit. Using the cDNA-AFLP technique, we screened more than 2500 transcript derived fragments, of which 33 (1.2%) showed clear variation in presence/absence between non stressed and stressed medium. The relative abundance of these transcripts was then analysed by reverse northern. Only two out of these 33 genes showed significant opposite behaviour between both techniques. The identification and characterization of water-deficit responsive genes in roots provide the emergence of physiological understanding of the patterns of gene expression and regulation involved in the drought stress response of maritime pine.