Proliferation and Apoptosis in Long-term Surviving Low Grade Gliomas in Relation to Radiotherapy
Proliferation and Apoptosis in Long-term Surviving Low Grade Gliomas in Relation to Radiotherapy
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长期存活的低级别胶质瘤的增殖和凋亡与放疗的关系
DOI：
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发表时间：
2002
影响因子：
3.9
通讯作者：
W. Molenaar
中科院分区：
文献类型：
--
作者：
M. Heesters;J. Koudstaal;K. Gwan Go;W. Molenaar
鉴别具有长期无复发生存期的低级别胶质瘤患者具有临床价值，因为放疗可以推迟到复发。与原发肿瘤相比，复发性胶质瘤的恶性程度可能增加，这可能与放疗有关。我们通过计数有丝分裂图和mb -1标记来研究48例低级别胶质瘤的增殖，通过TUNEL来研究细胞凋亡，通过免疫组织化学分析p53、p21、bcl-2和bax的表达来研究细胞周期调节相关蛋白的表达。术后无复发生存超过9年的星形细胞瘤（A, n = 14）和少突胶质细胞瘤（O, n = 4）的p53指数明显低于经组织病理学证实复发的A （n = 18）和O （n = 12）。此外，无复发A具有更高的p21指数。mb - li、TUNEL-LI、bcl-2和bax的表达差异无统计学意义。首先比较低级别胶质瘤及其相应的复发率（n = 30）。在未放疗的胶质瘤中（n = 15），原发肿瘤与复发肿瘤间有丝分裂率、TUNEL-LI、p53、p21、bcl-2、bax的表达均无差异。只有复发肿瘤的mb - li较高。在接受放射治疗的胶质瘤中（n = 15），除了复发肿瘤的有丝分裂数量较高外，这些参数在原始肿瘤和复发肿瘤之间没有差异。我们得出结论，低级别胶质瘤具有长期无复发生存的特点是p53蛋白表达低，而在a的情况下，p21指数较高。我们没有发现放疗与复发性胶质瘤细胞增殖、凋亡或细胞周期调节相关蛋白表达的改变有关的证据。
Identification of patients with a low grade glioma with a long-term recurrence-free survival is of clinical value as radiotherapy can be postponed until recurrence. The recurring glioma may increase in malignancy compared to the original tumor, which is possibly related to radiotherapy. We studied proliferation by counting mitotic figures and by MIB-1 labeling, apoptosis by TUNEL and expression of proteins related to cell cycle regulation by immunohistochemical analysis of p53, p21, bcl-2 and bax expression in 48 low grade gliomas. Astrocytomas (A, n = 14) and oligodendrogliomas (O, n = 4) with a recurrence-free survival of more than 9 years after surgery had a significantly lower p53 index compared to A (n = 18) and O (n = 12) with a histopathologically documented recurrence. Additionally, the recurrence-free A had a higher p21 index. No significant differences were observed in MIB-LI, TUNEL-LI, bcl-2 and bax expression. Initially low grade gliomas and their corresponding recurrences were compared (n = 30). In the gliomas without radiotherapy (n = 15), no differences in mitotic rate, TUNEL-LI, p53, p21, bcl-2 and bax expression were found between primary tumors and their recurrences. Only MIB-LI was higher in the recurrent tumors. In the gliomas with radiotherapy (n = 15) no differences were detected in these parameters between the original tumor and the recurrent tumor except for a higher number of mitoses in the recurrent tumors. We conclude that low grade gliomas with a long-term recurrence-free survival were characterized by a low p53 protein expression and, in the case of A, a higher p21 index. We found no evidence that radiotherapy is involved in changes of proliferation, apoptosis or expression of proteins related to cell cycle regulation in recurring gliomas.
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影响因子：
4.1
作者：
Broaddus, WC;Liu, Y;Fillmore, HL
通讯作者：
Fillmore, HL
p53 功能影响分割放射治疗对胶质母细胞瘤的效果。
DOI：
10.1016/s0360-3016(98)00393-9
发表时间：
1999
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Haas-Kogan,DA;Kogan,SS;Yount,G;Hsu,J;Haas,M;Deen,DF;Israel,MA
通讯作者：
Israel,MA
影响因子：
56.9
作者：
LOWE, SW;BODIS, S;JACKS, T
通讯作者：
JACKS, T
DOI：
10.1073/pnas.95.24.14453
发表时间：
1998-11-24
影响因子：
11.1
作者：
Shu, HKG;Kim, MM;Israel, MA
通讯作者：
Israel, MA