High-throughput microfluidics: improved sample treatment and washing over standard wells
High-throughput microfluidics: improved sample treatment and washing over standard wells
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DOI：
10.1039/b613350a
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
--
作者：
Warrick, Jay;Meyvantsson, Ivar;Beebe, David J.
微通道中的流体流动用于处理或清洗样品，并且可以纳入高通量应用，例如药物筛选，目前使用标准微量滴定孔来进行测定。本文提供了比较微通道和标准孔在样品洗涤指标和处理浓度实验误差方面的理论和实验数据。数值和实验表明，微通道浓度可以通过与输入体积的反线性关系来近似。实验支持的数学近似和误差传播方法用于比较微通道与标准孔中处理的准确性和精密度。数学结果表明，对于高通量筛选的典型体积比，微通道的处理精度可以达到标准孔的 10 倍或更多倍。在处理-等待-处理方法中，利用被动泵和扩散来进一步提高微通道的准确度和精密度。这里概述的微通道的优点可以对筛选应用的成本和准确性产生大规模影响。
Fluid flow in microchannels is used to treat or wash samples and can be incorporated into high-throughput applications such as drug screening, which currently use standard microtiter wells for performing assays. This paper provides theoretical and experimental data comparing microchannels and standard wells on the metrics of sample washing and experimental error in treatment concentrations. It is shown numerically and experimentally that microchannel concentration can be approximated with an inverse linear relationship to input volume. The experimentally supported mathematical approximation and error propagation methods are used to compare the accuracy and precision of treatments in microchannels vs. standard wells. Mathematical results suggest microchannels can provide 10 or more times the treatment precision of standard wells for volume ratios typical of high-throughput screening. Passive-pumping and diffusion are utilized to improve microchannel accuracy and precision even further in a treat-wait-treat method. The advantages of microchannels outlined here can have large-scale effects on cost and accuracy in screening applications.